H2A.Z Has a Function Reminiscent of an Activator Required for Preferential Binding to Intergenic DNA

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 2003 Aug 28

PMID 12941702

Citations 37

Authors

Marc Larochelle

Luc Gaudreau

Affiliations

Soon will be listed here.

Abstract

H2A.Z has been shown to regulate transcription in yeast, and that function resides in its C-terminal region as the reciprocal portion of H2A cannot substitute for the latter. We show that fusion of a transcriptional activating region to the C-terminal region of H2A, which is substituted for that of H2A.Z, can allow the chimera to fulfil the special role of H2A.Z in positive gene regulation, as well as complement growth deficiencies of htz1delta cells. We further show that the 'transcription' function of H2A.Z is linked to its ability to preferentially localize to certain intergenic DNA regions. Our results suggest that H2A.Z modulates functional interactions with transcription regulatory components, and thus increases its localization to promoters where it helps poise chromatin for gene activation.

Citing Articles

Characterizing the regulatory effects of H2A.Z and SWR1-C on gene expression during hydroxyurea exposure in Saccharomyces cerevisiae.

Brewis H, Stirling P, Kobor M PLoS Genet. 2025; 21(1):e1011566.

PMID: 39836664 PMC: 11761084. DOI: 10.1371/journal.pgen.1011566.

The Histone Variant H2A.Z C-Terminal Domain Has Locus-Specific Differential Effects on H2A.Z Occupancy and Nucleosome Localization.

Neumann H, Jeronimo C, Lucier J, Pasquier E, Robert F, Wellinger R Microbiol Spectr. 2023; :e0255022.

PMID: 36815792 PMC: 10100702. DOI: 10.1128/spectrum.02550-22.

What makes a histone variant a variant: Changing H2A to become H2A.Z.

Brewis H, Wang A, Gaub A, Lau J, Stirling P, Kobor M PLoS Genet. 2021; 17(12):e1009950.

PMID: 34871303 PMC: 8675926. DOI: 10.1371/journal.pgen.1009950.

Genome-wide identification of histone H2A and histone variant H2A.Z-interacting proteins by bPPI-seq.

Zhang Y, Ku W, Liu S, Cui K, Jin W, Tang Q Cell Res. 2017; 27(10):1258-1274.

PMID: 28862252 PMC: 5630678. DOI: 10.1038/cr.2017.112.

Molecular basis and specificity of H2A.Z-H2B recognition and deposition by the histone chaperone YL1.

Latrick C, Marek M, Ouararhni K, Papin C, Stoll I, Ignatyeva M Nat Struct Mol Biol. 2016; 23(4):309-16.

PMID: 26974126 DOI: 10.1038/nsmb.3189.

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