Secreted Portion of Glycoprotein G of Herpes Simplex Virus Type 2 is a Novel Antigen for Type-discriminating Serology

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 2003 Aug 9

PMID 12904375

Citations 10

Authors

Staffan Gorander

Bo Svennerholm

Jan-Ake Liljeqvist

Affiliations

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Abstract

The secreted portion of glycoprotein G (sgG-2) of herpes simplex virus type 2 (HSV-2) was evaluated as a novel antigen in an enzyme-linked immunosorbent assay (ELISA) format for detection of type-specific immunoglobulin G (IgG) antibodies in HSV-2-infected patients. The results were compared with those obtained by a commercially available assay, the HerpeSelect 2 ELISA (the FOCUS2 assay). Five different panels of sera were analyzed: panel A consisted of 109 serum samples from patients with a culture-proven HSV-1 infection that were Western blotting (WB) negative for HSV-2; panel B consisted of 106 serum samples from patients with a culture-proven recurrent HSV-2 infection that were WB positive for HSV-2; panel C consisted of 100 serum samples with no detectable IgG antibodies against HSV-1 and HSV-2; panel D consisted of 70 HSV-2 negative "tricky" serum samples containing antinuclear IgG antibodies or IgM antibodies against other viruses or bacteria; and panel E consisted of consecutive serum samples from 21 patients presenting with a first episode of HSV-2-induced lesions. When sera in panels A to C were analyzed, the sgG-2 ELISA and the FOCUS2 assay both showed sensitivities and specificities of >or=98%. In total, among the samples in panel D, 13 serum samples (19%) were false positive by the FOCUS2 assay and 1 serum sample (1.4%) was false positive by the sgG-2 ELISA. When the sera in panel E were analyzed, the sgG-2 ELISA detected seroconversion somewhat later than WB or the FOCUS2 assay did. We conclude that sgG-2 induces an HSV-2 type-specific antibody response and can be used for type-discriminating serology.

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References

van Loon A, van der Logt J, Heessen F, van der Veen J . Use of enzyme-labeled antigen for the detection of immunoglobulin M and A antibody to herpes simplex virus in serum and cerebrospinal fluid. J Med Virol. 1985; 15(2):183-95. DOI: 10.1002/jmv.1890150211. View

Balachandran N, Hutt-Fletcher L . Synthesis and processing of glycoprotein gG of herpes simplex virus type 2. J Virol. 1985; 54(3):825-32. PMC: 254870. DOI: 10.1128/JVI.54.3.825-832.1985. View

Lee F, Coleman R, Pereira L, Bailey P, Tatsuno M, Nahmias A . Detection of herpes simplex virus type 2-specific antibody with glycoprotein G. J Clin Microbiol. 1985; 22(4):641-4. PMC: 268483. DOI: 10.1128/jcm.22.4.641-644.1985. View

Ashley R, Militoni J, Lee F, Nahmias A, Corey L . Comparison of Western blot (immunoblot) and glycoprotein G-specific immunodot enzyme assay for detecting antibodies to herpes simplex virus types 1 and 2 in human sera. J Clin Microbiol. 1988; 26(4):662-7. PMC: 266403. DOI: 10.1128/jcm.26.4.662-667.1988. View

Ashley R . Sorting out the new HSV type specific antibody tests. Sex Transm Infect. 2001; 77(4):232-7. PMC: 1744343. DOI: 10.1136/sti.77.4.232. View

Liljeqvist J, Trybala E, Hoebeke J, Svennerholm B, Bergstrom T . Monoclonal antibodies and human sera directed to the secreted glycoprotein G of herpes simplex virus type 2 recognize type-specific antigenic determinants. J Gen Virol. 2001; 83(Pt 1):157-165. DOI: 10.1099/0022-1317-83-1-157. View

Wald A, Link K . Risk of human immunodeficiency virus infection in herpes simplex virus type 2-seropositive persons: a meta-analysis. J Infect Dis. 2002; 185(1):45-52. DOI: 10.1086/338231. View

Ikoma M, Liljeqvist J, Groen J, Glazenburg K, The T, Welling-Wester S . Use of a fragment of glycoprotein G-2 produced in the baculovirus expression system for detecting herpes simplex virus type 2-specific antibodies. J Clin Microbiol. 2002; 40(7):2526-32. PMC: 120576. DOI: 10.1128/JCM.40.7.2526-2532.2002. View

Hogrefe W, Su X, Song J, Ashley R, Kong L . Detection of herpes simplex virus type 2-specific immunoglobulin G antibodies in African sera by using recombinant gG2, Western blotting, and gG2 inhibition. J Clin Microbiol. 2002; 40(10):3635-40. PMC: 130895. DOI: 10.1128/JCM.40.10.3635-3640.2002. View

10.

Jeansson S, Molin L . On the occurrence of genital herpes simplex virus infection. Clinical and virological findings and relation to gonorrhoea. Acta Derm Venereol. 1974; 54(6):479-85. View

11.

Jeansson S, Forsgren M, Svennerholm B . Evaluation of solubilized herpes simplex virus membrane antigen by enzyme-linked immunosorbent assay. J Clin Microbiol. 1983; 18(5):1160-6. PMC: 272860. DOI: 10.1128/jcm.18.5.1160-1166.1983. View

12.

Svennerholm B, Olofsson S, Jeansson S, Vahlne A, LYCKE E . Herpes simplex virus type-selective enzyme-linked immunosorbent assay with Helix pomatia lectin-purified antigens. J Clin Microbiol. 1984; 19(2):235-9. PMC: 271028. DOI: 10.1128/jcm.19.2.235-239.1984. View

13.

Nahmias A, Lee F . Sero-epidemiological and -sociological patterns of herpes simplex virus infection in the world. Scand J Infect Dis Suppl. 1990; 69:19-36. View

14.

Brown Z, Benedetti J, Ashley R, Burchett S, Selke S, Berry S . Neonatal herpes simplex virus infection in relation to asymptomatic maternal infection at the time of labor. N Engl J Med. 1991; 324(18):1247-52. DOI: 10.1056/NEJM199105023241804. View

15.

Ho D, Field P, Sjogren-Jansson E, Jeansson S, Cunningham A . Indirect ELISA for the detection of HSV-2 specific IgG and IgM antibodies with glycoprotein G (gG-2). J Virol Methods. 1992; 36(3):249-64. DOI: 10.1016/0166-0934(92)90056-j. View

16.

Su H, Fetherston J, Smith M, Courtney R . Orientation of the cleavage site of the herpes simplex virus glycoprotein G-2. J Virol. 1993; 67(5):2954-9. PMC: 237626. DOI: 10.1128/JVI.67.5.2954-2959.1993. View

17.

Forsgren M, Skoog E, Jeansson S, Olofsson S, Giesecke J . Prevalence of antibodies to herpes simplex virus in pregnant women in Stockholm in 1969, 1983 and 1989: implications for STD epidemiology. Int J STD AIDS. 1994; 5(2):113-6. DOI: 10.1177/095646249400500207. View

18.

Beck E, Mandalia S, Leonard K, Griffith R, Harris J, Miller D . Case-control study of sexually transmitted diseases as cofactors for HIV-1 transmission. Int J STD AIDS. 1996; 7(1):34-8. DOI: 10.1258/0956462961917023. View

19.

Slomka M . Seroepidemiology and control of genital herpes: the value of type specific antibodies to herpes simplex virus. Commun Dis Rep CDR Rev. 1996; 6(3):R41-5. View

20.

Mindel A . Psychological and psychosexual implications of herpes simplex virus infections. Scand J Infect Dis Suppl. 1996; 100:27-32. View