The Three Extra-cellular Zinc Metalloproteinases of Streptococcus Pneumoniae Have a Different Impact on Virulence in Mice

Overview

Journal BMC Microbiol

Publisher Biomed Central

Specialty Microbiology

Date 2003 Jul 5

PMID 12841855

Citations 31

Authors

Damiana Chiavolini

Guido Memmi

Tiziana Maggi

Francesco Iannelli

Gianni Pozzi

Marco R Oggioni

Affiliations

Soon will be listed here.

Abstract

Background: Streptococcus pneumoniae possesses large zinc metalloproteinases on its surface. To analyse the importance in virulence of three of these metalloproteinases, intranasal challenge of MF1 outbred mice was carried out using a range of infecting doses of wild type and knock-out pneumococcal mutant strains, in order to compare mice survival.

Results: Observation of survival percentages over time and detection of LD50s of knock out mutants in the proteinase genes in comparison to the type 4 TIGR4 wild type strain revealed two major aspects: i) Iga and ZmpB, present in all strains of S. pneumoniae, strongly contribute to virulence in mice; (ii) ZmpC, only present in about 25% of pneumococcal strains, has a lower influence on virulence in mice.

Conclusions: These data suggest Iga, ZmpB and ZmpC as candidate surface proteins responsible for pneumococcal infection and potentially involved in distinct stages of pneumococcal disease.

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