Synergistic Antimicrobial Activity of Metabolites Produced by a Nonobligate Bacterial Predator

Overview

Journal Antimicrob Agents Chemother

Publisher American Society for Microbiology

Specialty Pharmacology

Date 2003 Jun 25

PMID 12821455

Citations 24

Authors

Cody C Cain

Dongho Lee

Robert H Waldo 3rd

Alexis T Henry

Earl J Casida Jr

Mansukh C Wani

Monroe E Wall

Nicholas H Oberlies

Joseph O Falkinham 3rd

Affiliations

Soon will be listed here.

Abstract

A naturally occurring, gram-negative, nonobligate predator bacterial strain 679-2, exhibits broad-spectrum antimicrobial activity that is due, in part, to the production of three extracellular compounds. Antimicrobial-activity-directed fractionation of a culture of strain 679-2 against a panel of microorganisms has led to the isolation of three compounds: pyrrolnitrin, maculosin, and a new compound, which we have named banegasine. Although pyrrolnitrin is well known in the literature, it has not been found in cells with the herbicide maculosin. Further, this is the first report of production of maculosin by a prokaryote. Both maculosin and banegasine, which displayed no antimicrobial activities alone, were found to potentiate the antimicrobial activity of pyrrolnitrin. Based on 16S rRNA sequence, cellular fatty acid composition, and biochemical and cultural characteristics, strain 679-2 appears to represent a new genus and species of eubacteria, Aristabacter necator. The potent, broad-spectrum antimicrobial activity of predator strain 679-2 may be due to synergism between metabolites.

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References

Kuntz I . Structure-based strategies for drug design and discovery. Science. 1992; 257(5073):1078-82. DOI: 10.1126/science.257.5073.1078. View

Davies J . Inactivation of antibiotics and the dissemination of resistance genes. Science. 1994; 264(5157):375-82. DOI: 10.1126/science.8153624. View

Shu Y . Recent natural products based drug development: a pharmaceutical industry perspective. J Nat Prod. 1998; 61(8):1053-71. DOI: 10.1021/np9800102. View

Breithaupt H . The new antibiotics. Nat Biotechnol. 1999; 17(12):1165-9. DOI: 10.1038/70705. View

Cavalieri S, Biehle J, Sanders Jr W . Synergistic activities of clarithromycin and antituberculous drugs against multidrug-resistant Mycobacterium tuberculosis. Antimicrob Agents Chemother. 1995; 39(7):1542-5. PMC: 162778. DOI: 10.1128/AAC.39.7.1542. View

Neu H . The crisis in antibiotic resistance. Science. 1992; 257(5073):1064-73. DOI: 10.1126/science.257.5073.1064. View

van Pee K, Ligon J . Biosynthesis of pyrrolnitrin and other phenylpyrrole derivatives by bacteria. Nat Prod Rep. 2000; 17(2):157-64. DOI: 10.1039/a902138h. View

Cragg G, Newman D . Natural product drug discovery in the next millennium. Pharm Biol. 2011; 39 Suppl 1:8-17. DOI: 10.1076/phbi.39.s1.8.0009. View

Sako M, Kihara T, Okada K, Ohtani Y, Kawamoto H . Reductive cleavage of heteroaryl c-halogen bonds by iodotrimethylsilane. Facile and regioselective dechlorination of antibiotic pyrrolnitrin. J Org Chem. 2001; 66(10):3610-2. DOI: 10.1021/jo001644s. View

10.

Thomashow L, Weller D, Bonsall R, Pierson L . Production of the antibiotic phenazine-1-carboxylic Acid by fluorescent pseudomonas species in the rhizosphere of wheat. Appl Environ Microbiol. 1990; 56(4):908-12. PMC: 184320. DOI: 10.1128/aem.56.4.908-912.1990. View

11.

Arima K, Imanaka H, Kousaka M, Fukuda A, Tamura G . Studies on pyrrolnitrin, a new antibiotic. I. Isolation and properties of pyrrolnitrin. J Antibiot (Tokyo). 1965; 18(5):201-4. View

12.

Stierle A, Cardellina J, Strobel G . Maculosin, a host-specific phytotoxin for spotted knapweed from Alternaria alternata. Proc Natl Acad Sci U S A. 1988; 85(21):8008-11. PMC: 282343. DOI: 10.1073/pnas.85.21.8008. View

13.

Stermitz F, Lorenz P, Tawara J, Zenewicz L, Lewis K . Synergy in a medicinal plant: antimicrobial action of berberine potentiated by 5'-methoxyhydnocarpin, a multidrug pump inhibitor. Proc Natl Acad Sci U S A. 2000; 97(4):1433-7. PMC: 26451. DOI: 10.1073/pnas.030540597. View

14.

Hill D, Stein J, Torkewitz N, Morse A, Howell C, Pachlatko J . Cloning of Genes Involved in the Synthesis of Pyrrolnitrin from Pseudomonas fluorescens and Role of Pyrrolnitrin Synthesis in Biological Control of Plant Disease. Appl Environ Microbiol. 1994; 60(1):78-85. PMC: 201272. DOI: 10.1128/aem.60.1.78-85.1994. View

15.

BERENBAUM M . Correlations between methods for measurement of synergy. J Infect Dis. 1980; 142(3):476-80. DOI: 10.1093/infdis/142.3.476. View

16.

CAIN C, Henry A, Waldo 3rd R, Casida Jr L, Falkinham 3rd J . Identification and characteristics of a novel Burkholderia strain with broad-spectrum antimicrobial activity. Appl Environ Microbiol. 2000; 66(9):4139-41. PMC: 92273. DOI: 10.1128/AEM.66.9.4139-4141.2000. View

17.

Cohen M . Epidemiology of drug resistance: implications for a post-antimicrobial era. Science. 1992; 257(5073):1050-5. DOI: 10.1126/science.257.5073.1050. View

18.

Winkelmann G . Pyrrolnitrin from Burkholderia cepacia: antibiotic activity against fungi and novel activities against streptomycetes. J Appl Microbiol. 1998; 85(1):69-78. DOI: 10.1046/j.1365-2672.1998.00473.x. View

19.

DHOPLE A, Ibanez M, Gardner G . In vitro synergistic activity between ofloxacin and ansamycins against Mycobacterium leprae. Arzneimittelforschung. 1993; 43(3):384-6. View

20.

Jewell S, Waldo 3rd R, Cain C, Falkinham 3rd J . Rapid detection of lytic antimicrobial activity against yeast and filamentous fungi. J Microbiol Methods. 2002; 49(1):1-9. DOI: 10.1016/s0167-7012(01)00360-8. View