Development of PCR-based Markers Linked to Dominant Genes for Male-fertility Restoration in Pampa CMS of Rye (Secale Cereale L.)

Overview

Journal Theor Appl Genet

Publisher Springer

Specialty Genetics

Date 2003 May 16

PMID 12748768

Citations 13

Authors

S Stracke

A G Schilling

J Forster

C Weiss

C Glass

T Miedaner

H H Geiger

Affiliations

Soon will be listed here.

Abstract

Cytoplasmatic male sterility (CMS) is the basis for commercial hybrid seed production of rye. Nuclear restorer genes are indispensable for a complete restoration of fertility of the CMS lines. The drawbacks of current European restorer lines require the utilisation of new genetic resources that have been recently detected in an Iranian primitive rye population (IRAN IX) and an Argentinean landrace (Pico Gentario). The introgression of these effective restorer genes (Rfp1 and Rfp2, respectively) into breeding material can be facilitated by marker-assisted selection. Using two F(2) populations based on crosses between the non-restorer inbred line Lo6 and the restorer IRAN IX, as well as Pico Gentario, RAPDs and AFLPs were screened and led to a closely linked marker set for each of these genes. The conversion of the closest markers into fragment-specific sequence-characterised amplified region (SCAR) markers resulted in flanking ranges of 2.9 cM (Rfp1) and 5.2 cM (Rfp2). The application of these markers in backcross programmes is discussed.

Citing Articles

Improving Yield and Yield Stability in Winter Rye by Hybrid Breeding.

Hackauf B, Siekmann D, Fromme F Plants (Basel). 2022; 11(19).

PMID: 36235531 PMC: 9571156. DOI: 10.3390/plants11192666.

Effective Pollen-Fertility Restoration Is the Basis of Hybrid Rye Production and Ergot Mitigation.

Miedaner T, Korzun V, Wilde P Plants (Basel). 2022; 11(9).

PMID: 35567115 PMC: 9104404. DOI: 10.3390/plants11091115.

Genomic Scan of Male Fertility Restoration Genes in a 'Gülzow' Type Hybrid Breeding System of Rye ( L.).

Vendelbo N, Mahmood K, Sarup P, Kristensen P, Orabi J, Jahoor A Int J Mol Sci. 2021; 22(17).

PMID: 34502186 PMC: 8431178. DOI: 10.3390/ijms22179277.

New PCR-specific markers for pollen fertility restoration QRfp-4R in rye (Secale cereale L.) with Pampa sterilizing cytoplasm.

Niedziela A, Wojciechowska M, Bednarek P J Appl Genet. 2021; 62(4):545-557.

PMID: 34173177 PMC: 8571214. DOI: 10.1007/s13353-021-00646-z.

Genetic mapping of pollen fertility restoration QTLs in rye (Secale cereale L.) with CMS Pampa.

Niedziela A, Brukwinski W, Bednarek P J Appl Genet. 2021; 62(2):185-198.

PMID: 33409933 PMC: 8032618. DOI: 10.1007/s13353-020-00599-9.

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