Genetic Mapping of Pollen Fertility Restoration QTLs in Rye (Secale Cereale L.) with CMS Pampa

Overview

Journal J Appl Genet

Publisher Springer

Specialty Genetics

Date 2021 Jan 7

PMID 33409933

Citations 2

Authors

Agnieszka Niedziela

Waldemar Brukwinski

Piotr Tomasz Bednarek

Affiliations

Soon will be listed here.

Abstract

Cytoplasmic male sterility (CMS) is a widely applied plant breeding tool for hybrid seed production. The phenomenon is often caused by chimeric genes with altered open reading frames (ORFs) located in the mitochondrial genomes and expressed as novel genotoxic products that induce pollen abortion. The fertility of CMS plants can be restored by nuclear-encoded genes that inhibit the action of ORFs responsible for pollen sterility. A recombinant inbred line (RIL) mapping population S64/04/01, encompassing 175 individuals, was used for genetic map construction and identification of quantitative trait loci (QTLs) responsible for fertility restoration in rye (Secale cereale L.) with CMS Pampa. The genetic map of all seven rye chromosomes included 15,516 SNP and silicoDArT markers and covered 1070.5 cm. Individual QTLs explaining 60% and 5.5% of the fertility trait's phenotypic variance were mapped to chromosomes 4R (QRft-4R) and 5R (QRft-5R), respectively. Association mapping identified markers with the highest R value of 0.58 (p value = 2.21E-28). Markers showing the highest associations with the trait were also mapped to the 4R chromosome within the QRft-4R region. Based on marker sequence homology, putative genes involved in pollen fertility restoration were suggested. Five silicoDArTs were converted into PCR-based markers for further breeding purposes.

Citing Articles

Genomic Scan of Male Fertility Restoration Genes in a 'Gülzow' Type Hybrid Breeding System of Rye ( L.).

Vendelbo N, Mahmood K, Sarup P, Kristensen P, Orabi J, Jahoor A Int J Mol Sci. 2021; 22(17).

PMID: 34502186 PMC: 8431178. DOI: 10.3390/ijms22179277.

New PCR-specific markers for pollen fertility restoration QRfp-4R in rye (Secale cereale L.) with Pampa sterilizing cytoplasm.

Niedziela A, Wojciechowska M, Bednarek P J Appl Genet. 2021; 62(4):545-557.

PMID: 34173177 PMC: 8571214. DOI: 10.1007/s13353-021-00646-z.

References

Wang Z, Zou Y, Li X, Zhang Q, Chen L, Wu H . Cytoplasmic male sterility of rice with boro II cytoplasm is caused by a cytotoxic peptide and is restored by two related PPR motif genes via distinct modes of mRNA silencing. Plant Cell. 2006; 18(3):676-87. PMC: 1383642. DOI: 10.1105/tpc.105.038240. View

Bradbury P, Zhang Z, Kroon D, Casstevens T, Ramdoss Y, Buckler E . TASSEL: software for association mapping of complex traits in diverse samples. Bioinformatics. 2007; 23(19):2633-5. DOI: 10.1093/bioinformatics/btm308. View

Milczarski P, Hanek M, Tyrka M, Stojalowski S . The application of GBS markers for extending the dense genetic map of rye (Secale cereale L.) and the localization of the Rfc1 gene restoring male fertility in plants with the C source of sterility-inducing cytoplasm. J Appl Genet. 2016; 57(4):439-451. PMC: 5061839. DOI: 10.1007/s13353-016-0347-4. View

Miedaner T, Geiger H . Biology, genetics, and management of ergot (Claviceps spp.) in rye, sorghum, and pearl millet. Toxins (Basel). 2015; 7(3):659-78. PMC: 4379517. DOI: 10.3390/toxins7030659. View

Uyttewaal M, Arnal N, Quadrado M, Martin-Canadell A, Vrielynck N, Hiard S . Characterization of Raphanus sativus pentatricopeptide repeat proteins encoded by the fertility restorer locus for Ogura cytoplasmic male sterility. Plant Cell. 2008; 20(12):3331-45. PMC: 2630448. DOI: 10.1105/tpc.107.057208. View

Small I, Schallenberg-Rudinger M, Takenaka M, Mireau H, Ostersetzer-Biran O . Plant organellar RNA editing: what 30 years of research has revealed. Plant J. 2019; 101(5):1040-1056. DOI: 10.1111/tpj.14578. View

Melz G, Adolf K . Genetic analysis of rye (Secale cereale L.) Genetics of male sterility of the G-type. Theor Appl Genet. 2013; 82(6):761-4. DOI: 10.1007/BF00227322. View

Myskow B, Hanek M, Banek-Tabor A, Maciorowski R, Stojalowski S . The application of high-density genetic maps of rye for the detection of QTLs controlling morphological traits. J Appl Genet. 2013; 55(1):15-26. PMC: 3909618. DOI: 10.1007/s13353-013-0186-5. View

Bolibok-Bragoszewska H, Heller-Uszynska K, Wenzl P, Uszynski G, Kilian A, Rakoczy-Trojanowska M . DArT markers for the rye genome - genetic diversity and mapping. BMC Genomics. 2009; 10:578. PMC: 2795769. DOI: 10.1186/1471-2164-10-578. View

10.

Voorrips R . MapChart: software for the graphical presentation of linkage maps and QTLs. J Hered. 2002; 93(1):77-8. DOI: 10.1093/jhered/93.1.77. View

11.

Bauer E, Schmutzer T, Barilar I, Mascher M, Gundlach H, Martis M . Towards a whole-genome sequence for rye (Secale cereale L.). Plant J. 2016; 89(5):853-869. DOI: 10.1111/tpj.13436. View

12.

Bernhard T, Koch M, Snowdon R, Friedt W, Wittkop B . Undesired fertility restoration in msm1 barley associates with two mTERF genes. Theor Appl Genet. 2019; 132(5):1335-1350. DOI: 10.1007/s00122-019-03281-9. View

13.

Kazama T, Toriyama K . A pentatricopeptide repeat-containing gene that promotes the processing of aberrant atp6 RNA of cytoplasmic male-sterile rice. FEBS Lett. 2003; 544(1-3):99-102. DOI: 10.1016/s0014-5793(03)00480-0. View

14.

Milczarski P, Bolibok-Bragoszewska H, Myskow B, Stojalowski S, Heller-Uszynska K, Goralska M . A high density consensus map of rye (Secale cereale L.) based on DArT markers. PLoS One. 2011; 6(12):e28495. PMC: 3232230. DOI: 10.1371/journal.pone.0028495. View

15.

Cockram J, Mackay I . Genetic Mapping Populations for Conducting High-Resolution Trait Mapping in Plants. Adv Biochem Eng Biotechnol. 2018; 164:109-138. DOI: 10.1007/10_2017_48. View

16.

Brown G, Formanova N, Jin H, Wargachuk R, Dendy C, Patil P . The radish Rfo restorer gene of Ogura cytoplasmic male sterility encodes a protein with multiple pentatricopeptide repeats. Plant J. 2003; 35(2):262-72. DOI: 10.1046/j.1365-313x.2003.01799.x. View

17.

Bednarek P, Masojc P, Lewandowska R, Myskow B . Saturating rye genetic map with amplified fragment length polymorphism (AFLP) and random amplified polymorphic DNA (RAPD) markers. J Appl Genet. 2003; 44(1):21-33. View

18.

Geiger H, Morgenstern K . [Applied genetic studies on cytoplasmic pollen sterility in winter rye]. Theor Appl Genet. 2014; 46(6):269-76. DOI: 10.1007/BF00281148. View

19.

Zhao Y, Cai M, Zhang X, Li Y, Zhang J, Zhao H . Genome-wide identification, evolution and expression analysis of mTERF gene family in maize. PLoS One. 2014; 9(4):e94126. PMC: 3981765. DOI: 10.1371/journal.pone.0094126. View

20.

Stojalowski S, Milczarski P, Hanek M, Bolibok-Bragoszewska H, Myskow B, Kilian A . DArT markers tightly linked with the Rfc1 gene controlling restoration of male fertility in the CMS-C system in cultivated rye (Secale cereale L.). J Appl Genet. 2011; 52(3):313-8. PMC: 3132309. DOI: 10.1007/s13353-011-0049-x. View