Further Studies on the Ribosomal RNA Cistrons of Sciara Coprophila (Diptera)

Overview

Journal Genetics

Publisher Oxford University Press

Specialty Genetics

Date 1976 May 1

PMID 1269920

Citations 10

Authors

S A Gerbi

H V CROUSE

Affiliations

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Abstract

Additional experiments with homologous as well as heterologous hybridization confirmed our previous finding in Sciara coprophila that XX females have nearly twice the number of ribosomal RNA cistrons as XO males. A comparison between two different X' chromosomes revealed that only the one carrying the irradiation-induced Wavy mutation has a deletion of 70% of its ribosomal RNA cistrons as compared to the standard X. The deletion is relatively stable, and the remaining ribosomal RNA cistrons donot appear to undergo disproportionate replication or magnification as in Drosophila. Homologous hybridization experiments revealed an unusually low reiteration of ribosomal RNA cistrons in this fly, 45 gene copies per X chromosome. The question is raised as to whether such a low number of cistrons may be related to the unusual nucleolar condition encountered in the Sciaridae.

Citing Articles

Non-random chromosome segregation and chromosome eliminations in the fly Bradysia (Sciara).

Gerbi S Chromosome Res. 2022; 30(2-3):273-288.

PMID: 35793056 PMC: 10777868. DOI: 10.1007/s10577-022-09701-9.

Dramatic nucleolar dispersion in the salivary gland of Schwenkfeldina sp. (Diptera: Sciaridae).

Amabis J, Gorab E Sci Rep. 2021; 11(1):8347.

PMID: 33863925 PMC: 8052372. DOI: 10.1038/s41598-021-87012-5.

Molecular and cytological characterization of repetitive DNA sequences from the centromeric heterochromatin of Sciara coprophila.

Escriba M, Greciano P, Mendez-Lago M, de Pablos B, Trifonov V, Ferguson-Smith M Chromosoma. 2011; 120(4):387-97.

PMID: 21533987 DOI: 10.1007/s00412-011-0320-2.

Two new chromodomain-containing proteins that associate with heterochromatin in Sciara coprophila chromosomes.

Greciano P, Ruiz M, Kremer L, Goday C Chromosoma. 2009; 118(3):361-76.

PMID: 19205716 DOI: 10.1007/s00412-009-0203-y.

The localization of ribosomal DNA in Sciaridae (Diptera: Nematocera) reassessed.

Madalena C, Amabis J, Stocker A, Gorab E Chromosome Res. 2007; 15(4):409-16.

PMID: 17364224 DOI: 10.1007/s10577-007-1127-0.

References

Gambarini A, Lara F . Under-replication of ribosomal cistrons in polytene chromosomes of Rhynchosciara. J Cell Biol. 1974; 62(1):215-22. PMC: 2109172. DOI: 10.1083/jcb.62.1.215. View

Serfling E, Panitz R, Wobus U . Gel electrophoretic characterization of dipteran RNA. Acta Biol Med Ger. 1972; 29(4):509-19. View

Hennig W, MEER B . Reduced polyteny of ribosomal RNA cistrons in giant chromosomes of Drosophila hydei. Nat New Biol. 1971; 233(37):70-2. DOI: 10.1038/newbio233070a0. View

Miller L, Gurdon J . Mutations affecting the size of the nucleolus in Xenopus laevis. Nature. 1970; 227(5263):1108-10. DOI: 10.1038/2271108a0. View

Loening U . Molecular weights of ribosomal RNA in relation to evolution. J Mol Biol. 1968; 38(3):355-65. DOI: 10.1016/0022-2836(68)90391-4. View

GABRUSEWYCZ-GARCIA N, KLEINFELD R . A study of the nucleolar material in Sciara coprophila. J Cell Biol. 1966; 29(2):347-59. PMC: 2106905. DOI: 10.1083/jcb.29.2.347. View

ESPER H, BARR H . A STUDY OF THE DEVELOPMENTAL CYTOLOGY OF A MUTATION AFFECTING NUCLEOLI IN XENOPUS EMBRYOS. Dev Biol. 1964; 10:105-21. DOI: 10.1016/0012-1606(64)90007-7. View

METZ C, Smith H . Further Observations on the Nature of the X-Prime (X') Chromosome in Sciara. Proc Natl Acad Sci U S A. 1931; 17(4):195-8. PMC: 1076029. DOI: 10.1073/pnas.17.4.195. View

Spear B, Gall J . Independent control of ribosomal gene replication in polytene chromosomes of Drosophila melanogaster. Proc Natl Acad Sci U S A. 1973; 70(5):1359-63. PMC: 433497. DOI: 10.1073/pnas.70.5.1359. View

10.

Gerbi S . Localization and characterization of the ribosomal RNA cistrons in Sciara coprophila. J Mol Biol. 1971; 58(2):499-511. DOI: 10.1016/0022-2836(71)90367-6. View

11.

Miller L, Knowland J . Reduction of ribosomal RNA synthesis and ribosomal RNA genes in a mutant of Xenopus laevis which organizes only a partial nucleolus. II. The number of ribosomal RNA genes in animals of different nucleolar types. J Mol Biol. 1970; 53(3):329-38. DOI: 10.1016/0022-2836(70)90069-0. View

12.

Rieffel S, CROUSE H . The elimination and differentiation of chromosomes in the germ line of sciara. Chromosoma. 1966; 19(3):231-76. DOI: 10.1007/BF00326917. View

13.

Breuer M, Pavan C . Behavior of polytene chromosomes of Rhynchosciara angelae at different stages of larval development. Chromosoma. 1955; 7(4):341-86. View

14.

METZ C, Ullian S . GENETIC IDENTIFICATION OF THE SEX CHROMOSOMES IN SCIARA (DIPTERA). Proc Natl Acad Sci U S A. 1929; 15(2):82-5. PMC: 522409. DOI: 10.1073/pnas.15.2.82. View