MexXY-OprM Efflux Pump is Necessary for a Adaptive Resistance of Pseudomonas Aeruginosa to Aminoglycosides

Overview

Journal Antimicrob Agents Chemother

Publisher American Society for Microbiology

Specialty Pharmacology

Date 2003 Mar 26

PMID 12654672

Citations 74

Authors

Didier Hocquet

Christelle Vogne

Farid El Garch

Anne Vejux

Naomasa Gotoh

Angela Lee

Olga Lomovskaya

Patrick Plesiat

Affiliations

Soon will be listed here.

Abstract

Exposure of Pseudomonas aeruginosa to aminoglycosides frequently selects for recalcitrant subpopulations exhibiting an unstable, "adaptive" resistance to these antibiotics. In this study, we investigated the implication in the phenomenon of MexXY-OprM, an active efflux system known to export aminoglycosides in P. aeruginosa. Immunoblotting experiments demonstrated that the transporter MexY, but not the outer membrane pore OprM, was overproduced during the post-drug exposure adaptation period in wild-type strain PAO1. Furthermore, MexY production was dependent upon the degree of bacterial exposure to gentamicin (drug concentration). In contrast to parental strain PAO1, mutants defective in MexXY or in OprM were unable to develop adaptive resistance. Altogether, these results indicate that the resistance process requires the rapid production of MexXY and the interaction of these proteins with the constitutively produced component OprM.

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