Measurement of Vitamin E Metabolites by High-performance Liquid Chromatography During High-dose Administration of Alpha-tocopherol

Overview

Journal Eur J Clin Nutr

Specialty Nutritional Sciences

Date 2003 Mar 11

PMID 12627176

Citations 9

Authors

T Morinobu

S Yoshikawa

K Hamamura

H Tamai

Affiliations

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Abstract

Method: alpha-Tocopherol and gamma-tocopherol are metabolized into 2,5,7,8-tetramethyl-2-(2'-carboxyethyl)-6-hydroxychroman (alpha-CEHC) and 2,7,8-trimethyl-2-(2'-carboxyethyl)-6-hydroxychroman (gamma-CEHC), respectively. We analyzed alpha- and gamma-CEHC concentrations in human serum and urine by high-performance liquid chromatography during administration of alpha-tocopherol. Fourteen healthy adult male volunteers received 1,200 IU per day of RRR-alpha-tocopherol for 28 days. Blood and urine samples were obtained on days 0, 14, 28, and 56.

Results: During alpha-tocopherol administration, the plasma gamma-tocopherol concentration decreased significantly, but there was marked elevation of the alpha-tocopherol concentration. Increased concentration of alpha-CEHC and gamma-CEHC in both serum and urine indicated the acceleration of vitamin E metabolism.

Conclusion: High-dose administration of alpha-tocopherol caused an increase of gamma-tocopherol metabolism, which might have caused a decrease of the plasma gamma-tocopherol concentration.

Citing Articles

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Alpha-Tocopherol Metabolites (the Vitamin E Metabolome) and Their Interindividual Variability during Supplementation.

Bartolini D, Marinelli R, Giusepponi D, Galarini R, Barola C, Stabile A Antioxidants (Basel). 2021; 10(2).

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Circulating γ-Tocopherol Concentrations Are Inversely Associated with Antioxidant Exposures and Directly Associated with Systemic Oxidative Stress and Inflammation in Adults.

Abdulla K, Um C, Gross M, Bostick R J Nutr. 2018; 148(9):1453-1461.

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Schmolz L, Birringer M, Lorkowski S, Wallert M World J Biol Chem. 2016; 7(1):14-43.

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