Differentiation, Cell Fusion, and Nuclear Fusion During Ex Vivo Repair of Epithelium by Human Adult Stem Cells from Bone Marrow Stroma

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2003 Feb 28

PMID 12606728

Citations 168

Authors

Jeffrey L Spees

Scott D Olson

Joni Ylostalo

Patrick J Lynch

Jason Smith

Anthony Perry

Alexandra Peister

Meng Yu Wang

Darwin J Prockop

Affiliations

Soon will be listed here.

Abstract

To investigate stem cell differentiation in response to tissue injury, human mesenchymal stem cells (hMSCs) were cocultured with heat-shocked small airway epithelial cells. A subset of the hMSCs rapidly differentiated into epithelium-like cells, and they restored the epithelial monolayer. Immunocytochemistry and microarray analyses demonstrated that the cells expressed many genes characteristic of normal small airway epithelial cells. Some hMSCs differentiated directly after incorporation into the epithelial monolayer but other hMSCs fused with epithelial cells. Surprisingly, cell fusion was a frequent rather than rare event, in that up to 1% of the hMSCs added to the coculture system were recovered as binucleated cells expressing an epithelial surface epitope. Some of the fused cells also underwent nuclear fusion.

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