A Promoter Activity is Present in the DNA Sequence Corresponding to the Hepatitis C Virus 5' UTR

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 2003 Feb 13

PMID 12582247

Citations 25

Authors

Estelle Dumas

Cathy Staedel

Marie Colombat

Sandrine Reigadas

Sandrine Chabas

Therese Astier-Gin

Annie Cahour

Simon Litvak

Michel Ventura

Affiliations

Soon will be listed here.

Abstract

The hepatitis C virus (HCV) 5' untranslated region (UTR) has been extensively studied with regard to its internal ribosomal entry site (IRES) activity. In this work we present results suggesting the existence of a strong promoter activity carried by the DNA sequence corresponding to the HCV 5' UTR. This activity was not detected when the HCV 5' UTR sequence was replaced by HCV 3' UTR or poliovirus 5' UTR sequences. These results were further confirmed by using bicistronic constructions. We demonstrated the presence of an mRNA initiated in this 5' UTR sequence and located the initiation site by the 5' RACE method at nucleotide 67. Furthermore, northern experiments and flow cytometry analysis showed the unambiguous activity of such a promoter sequence in stably transfected cells. Our results strongly suggest that the data obtained using bicistronic DNA constructs carrying the HCV 5' UTR should be analyzed not only at the translational but also at the transcriptional level.

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