Quantification of Phosphatidic Acid and Lysophosphatidic Acid by HPLC with Evaporative Light-scattering Detection
Overview
Affiliations
Phosphatidic acid (PA) and lysophosphatidic acid (LPA) are lipids that regulate cellular processes. PA stimulates kinases and may play a role in exocytosis and membrane fusion. LPA can induce cell proliferation, platelet aggregation, and microfilament formation. Due to the growing interest in these lipids, rapid purification and quantification of these lipids is desirable. We now describe a method that utilizes one HPLC run to separate trace amounts of PA and LPA from large amounts of lipids found in cellular extracts. A two-pump HPLC with a solvent system consisting of chloroform, methanol, water, and ammonium hydroxide was employed to produce a reliable, efficient purification of the two lipids. Lipid mass was quantified by a sensitive evaporative light-scattering detector. Using this new method, insulin addition increased both PA (87%) and LPA (217%) mass in Xenopus laevis oocytes.
Tarannum N, Kumar D, Agrawal R J Cancer Prev. 2023; 28(2):31-39.
PMID: 37434795 PMC: 10331031. DOI: 10.15430/JCP.2023.28.2.31.
Lipid Signaling During Gamete Maturation.
Mostafa S, Nader N, Machaca K Front Cell Dev Biol. 2022; 10:814876.
PMID: 36204680 PMC: 9531329. DOI: 10.3389/fcell.2022.814876.
Extracellular Vesicles in Liquid Biopsies: Potential for Disease Diagnosis.
Liu J, Chen Y, Pei F, Zeng C, Yao Y, Liao W Biomed Res Int. 2021; 2021:6611244.
PMID: 33506022 PMC: 7814955. DOI: 10.1155/2021/6611244.
Detection of the Ovarian Cancer Biomarker Lysophosphatidic Acid in Serum.
Franier B, Thompson M Biosensors (Basel). 2020; 10(2).
PMID: 32075013 PMC: 7168251. DOI: 10.3390/bios10020013.
Regulation of Membrane Turnover by Phosphatidic Acid: Cellular Functions and Disease Implications.
Thakur R, Naik A, Panda A, Raghu P Front Cell Dev Biol. 2019; 7:83.
PMID: 31231646 PMC: 6559011. DOI: 10.3389/fcell.2019.00083.