Isolation and Characterization of Caulobacter Crecentus Bacteriophage Phi Cd1

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1976 Feb 1

PMID 1255848

Citations 20

Authors

D West

C Lagenaur

N Agabian

Affiliations

Soon will be listed here.

Abstract

A DNA-containing bacteriophage, phiCd1, was isolated from sewage and shown to infect both stalked and swarmer cells of Caulobacter crescentus strain CB13B1a. phiCd1 is a small, icosohedral bacteriophage, 60 nm in diameter, which possesses a short, noncontractile tail, 10 to 12 nm in length. The bacteriophage particle is composed of at least eight structural proteins. phiCd1 nucleic acid exists as a linear duplex of DNA as judged by: (i) thermal denaturation (Tm), (ii) CsCl density gradient centrifugation, and (iii) chemical analysis of its base composition. The DNA is 61% guanosine plus cytosine, has a buoyant density in CsCl of 1.721 +/- 0.001 g/cm3, and denatures sharply at 78.5 C in 0.1 SSC (standard saline citrate) buffer. The S20, w value for the DNA is 34.3 +/- 0.1S as compared with T7 DNA, indicating a molecular weight of about 29 x 10(6).

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References

Agabian-Keshishian N, Shapiro L . Bacterial differentiation and phage infection. Virology. 1971; 44(1):46-53. DOI: 10.1016/0042-6822(71)90151-6. View

Agabian-Keshishian N, Shapiro L . Stalked bacteria: properties of deoxriybonucleic acid bacteriophage phiCbK. J Virol. 1970; 5(6):795-800. PMC: 376072. DOI: 10.1128/JVI.5.6.795-800.1970. View

Wu R, Kaiser A . Mapping the 5'-terminal nucleotides of the DNA of bacteriophage lambda and related phages. Proc Natl Acad Sci U S A. 1967; 57(1):170-7. PMC: 335480. DOI: 10.1073/pnas.57.1.170. View

Studier F . Bacteriophage T7. Science. 1972; 176(4033):367-76. DOI: 10.1126/science.176.4033.367. View

Rahmsdorf H, PAI S, Ponta H, Herrlich P, Roskoski Jr R, Schweiger M . Protein kinase induction in Escherichia coli by bacteriophage T7. Proc Natl Acad Sci U S A. 1974; 71(2):586-9. PMC: 388053. DOI: 10.1073/pnas.71.2.586. View

Jollick J, Wright B . A flagella specific bacteriophage for caulobacter. J Gen Virol. 1974; 22(2):197-205. DOI: 10.1099/0022-1317-22-2-197. View

Sonenshein A, Roscoe D . The course of phage phi-e infection in sporulating cells of Bacillus subtilis strain 3610. Virology. 1969; 39(2):265-75. DOI: 10.1016/0042-6822(69)90047-6. View

Leighton S, Rubenstein I . Calibration of molecular weight scales for DNA. J Mol Biol. 1969; 46(2):313-28. DOI: 10.1016/0022-2836(69)90424-0. View

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

10.

POINDEXTER J, Hornack P, Armstrong P . Intracellular development of a large DNA bacteriophage lytic for Caulobacter crescentus. Arch Mikrobiol. 1967; 59(1):237-46. DOI: 10.1007/BF00406337. View

11.

Schmidt J, STANIER R . The development of cellular stalks in bacteria. J Cell Biol. 1966; 28(3):423-36. PMC: 2106941. DOI: 10.1083/jcb.28.3.423. View

12.

Schmidt J . Observations on the adsorption of Caulobacter bacteriophages containing ribonucleic acid. J Gen Microbiol. 1966; 45(2):347-53. DOI: 10.1099/00221287-45-2-347. View

13.

POINDEXTER J . BIOLOGICAL PROPERTIES AND CLASSIFICATION OF THE CAULOBACTER GROUP. Bacteriol Rev. 1964; 28:231-95. PMC: 441226. DOI: 10.1128/br.28.3.231-295.1964. View

14.

HOUWINK A . Caulobacter; its morphogenesis, taxonomy and parasitism. Antonie Van Leeuwenhoek. 1955; 21(1):49-64. DOI: 10.1007/BF02543799. View

15.

SCHILDKRAUT C, MARMUR J, Doty P . Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCl. J Mol Biol. 1962; 4:430-43. DOI: 10.1016/s0022-2836(62)80100-4. View

16.

LOWRY O, ROSEBROUGH N, FARR A, RANDALL R . Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1):265-75. View

17.

Kurn N, Shapiro L . Regulation of the Caulobacter cell cycle. Curr Top Cell Regul. 1975; 9:41-64. DOI: 10.1016/b978-0-12-152809-6.50009-3. View

18.

Shapiro L, Agabian-Keshishian N, Hirsch A, Rosen O . Effect of dibutyryladenosine 3':5'-cyclic monophosphate on growth and differentiation in Caulobacter crescentus. Proc Natl Acad Sci U S A. 1972; 69(5):1225-9. PMC: 426669. DOI: 10.1073/pnas.69.5.1225. View

19.

Leonard K, KLEINSCHMIDT A, Agabian-Keshishian N, Shapiro L, Maizel Jr J . Structural studies on the capsid of Caulobacter crescentus bacteriophage phiCbK. J Mol Biol. 1972; 71(2):201-16. DOI: 10.1016/0022-2836(72)90346-4. View

20.

Studier F, Maizel Jr J . T7-directed protein synthesis. Virology. 1969; 39(3):575-86. DOI: 10.1016/0042-6822(69)90105-6. View