Biotransformation of Trichloroethene by Pure Bacterial Cultures

Overview

Journal Folia Microbiol (Praha)

Publisher Springer

Specialty Microbiology

Date 2002 Dec 31

PMID 12503388

Authors

J Ruzicka

J Muller

D Vit

V Hutecka

J Hoffmann

H Datkova

M Nemec

Affiliations

Soon will be listed here.

Abstract

From natural samples 11 isolates able to remove trichloroethene (CCl2CHCl) from an aqueous environment were obtained which were capable of cometabolic degradation of CCl2CHCl by an enzyme system for phenol degradation. At an initial CCl2CHCl concentration of 1 mg/L, the resting cells of particular cultures degraded 33-94% CCl2CHCl during 1 d and their transformation capacity ranged from 0.3 to 3.1 mg CCl2CHCl per g organic fraction. An analysis of a mixed phenol-fed culture with an excellent trichloroethene-degrading ability found a markedly minority isolate represented in the consortium to be responsible for this property. This culture degraded CCl2CHCl even at a low inoculum concentration and attained a transformation capacity of 14.7 mg CCl2CHCl per g. The increase in chloride concentration after degradation was quantitative when compared with the decrease in organically bound chlorine. The degree of CCl2CHCl degradation was affected by Me2S2; this substance can significantly reduce the degrading ability of some tested cultures (> 60%); however, it does not cause this inhibition with others.

References

Vogel T, Criddle C, McCarty P . ES Critical Reviews: Transformations of halogenated aliphatic compounds. Environ Sci Technol. 2009; 21(8):722-36. DOI: 10.1021/es00162a001. View

Ishida H, Nakamura K . Trichloroethylene degradation by Ralstonia sp. KN1-10A constitutively expressing phenol hydroxylase: transformation products, NADH limitation, and product toxicity. J Biosci Bioeng. 2005; 89(5):438-45. DOI: 10.1016/s1389-1723(00)89093-3. View

Sun A, Wood T . Trichloroethylene degradation and mineralization by pseudomonads and Methylosinus trichosporium OB3b. Appl Microbiol Biotechnol. 1996; 45(1-2):248-56. DOI: 10.1007/s002530050679. View

Hopkins G, McCarty P . Field evaluation of in situ aerobic cometabolism of trichloroethylene and three dichloroethylene isomers using phenol and toluene as the primary substrates. Environ Sci Technol. 2012; 29(6):1628-37. DOI: 10.1021/es00006a029. View

Ensign S, Hyman M, Arp D . Cometabolic degradation of chlorinated alkenes by alkene monooxygenase in a propylene-grown Xanthobacter strain. Appl Environ Microbiol. 1992; 58(9):3038-46. PMC: 183045. DOI: 10.1128/aem.58.9.3038-3046.1992. View

Fries M, Forney L, Tiedje J . Phenol- and toluene-degrading microbial populations from an aquifer in which successful trichloroethene cometabolism occurred. Appl Environ Microbiol. 1997; 63(4):1523-30. PMC: 1389555. DOI: 10.1128/aem.63.4.1523-1530.1997. View

Scholler C, Molin S, Wilkins K . Volatile metabolites from some gram-negative bacteria. Chemosphere. 1997; 35(7):1487-95. DOI: 10.1016/s0045-6535(97)00209-9. View

Chang H, Alvarez-Cohen L . Transformation capacities of chlorinated organics by mixed cultures enriched on methane, propane, toluene, or phenol. Biotechnol Bioeng. 1995; 45(5):440-9. DOI: 10.1002/bit.260450509. View

Sun , Hong , Wood . Modeling trichloroethylene degradation by a recombinant pseudomonad expressing toluene ortho-monooxygenase in a fixed-film bioreactor . Biotechnol Bioeng. 1999; 59(1):40-51. DOI: 10.1002/(sici)1097-0290(19980705)59:1<40::aid-bit6>3.0.co;2-t. View

10.

Arciero D, Vannelli T, Logan M, Hooper A . Degradation of trichloroethylene by the ammonia-oxidizing bacterium Nitrosomonas europaea. Biochem Biophys Res Commun. 1989; 159(2):640-3. DOI: 10.1016/0006-291x(89)90042-9. View

11.

Kyung K, Fleming H . Antimicrobial activity of sulfur compounds derived from cabbage. J Food Prot. 1997; 60(1):67-71. DOI: 10.4315/0362-028x-60.1.67. View

12.

Shih C, Davey M, Zhou J, Tiedje J, Criddle C . Effects of phenol feeding pattern on microbial community structure and cometabolism of trichloroethylene. Appl Environ Microbiol. 1996; 62(8):2953-60. PMC: 1388920. DOI: 10.1128/aem.62.8.2953-2960.1996. View

13.

Dabrock B, Riedel J, Bertram J, Gottschalk G . Isopropylbenzene (cumene)--a new substrate for the isolation of trichloroethene-degrading bacteria. Arch Microbiol. 1992; 158(1):9-13. DOI: 10.1007/BF00249058. View

14.

Tomita B, Inoue H, Chaya K, Nakamura A, Hamamura N, Ueno K . Identification of dimethyl disulfide-forming bacteria isolated from activated sludge. Appl Environ Microbiol. 1987; 53(7):1541-7. PMC: 203907. DOI: 10.1128/aem.53.7.1541-1547.1987. View

15.

Saeki H, Akira M, Furuhashi K, Averhoff B, Gottschalk G . Degradation of trichloroethene by a linear-plasmid-encoded alkene monooxygenase in Rhodococcus corallinus (Nocardia corallina) B-276. Microbiology (Reading). 1999; 145 ( Pt 7):1721-1730. DOI: 10.1099/13500872-145-7-1721. View

16.

Wilson J, Wilson B . Biotransformation of trichloroethylene in soil. Appl Environ Microbiol. 1985; 49(1):242-3. PMC: 238380. DOI: 10.1128/aem.49.1.242-243.1985. View

17.

Folsom B, Chapman P, Pritchard P . Phenol and trichloroethylene degradation by Pseudomonas cepacia G4: kinetics and interactions between substrates. Appl Environ Microbiol. 1990; 56(5):1279-85. PMC: 184395. DOI: 10.1128/aem.56.5.1279-1285.1990. View