Alpha 2.0
Login Register
» Articles » PMID: 12486222

Photoresponsive Polymer-enzyme Switches

Overview
Journal Proc Natl Acad Sci U S A
Specialty Science
Date 2002 Dec 18
PMID 12486222
Citations 35
Authors
Tsuyoshi Shimoboji
Edmund Larenas
Tim Fowler
Samarth Kulkarni
Allan S Hoffman
Patrick S Stayton
Affiliations
Soon will be listed here.
Abstract

The ability to photoregulate enzyme activities could provide important new opportunities for development of diagnostic assays, sequential bioprocessing, and lab assays in both traditional and microfluidic formats. We show here that the photoinduced changes in the size and hydration of a "smart" polymer chain coil can be used to regulate substrate access and enzyme activity when conjugated to the enzyme at a specific point just outside the active site. The photoresponsive polymers thus serve jointly as antennae and actuators that reversibly respond to distinct optical signals to switch the polymer-enzyme conjugates on and off, and work when the conjugate is free in solution or when immobilized on magnetic beads.

Citing Articles

Crystal Structure Landscape of Diarylethene-Based Crystalline Solids: A Comprehensive CSD Analysis.

Zhang X, Mitchell T, Benedict J Cryst Growth Des. 2024; 24(15):6284-6291.

PMID: 39131448 PMC: 11313429. DOI: 10.1021/acs.cgd.4c00556.

Reversible Solubility Switching of a Polymer Triggered by Visible-Light Responsive Azobenzene Photochromism with Negligible Thermal Relaxation.

Ueki T, Osaka Y, Homma K, Yamamoto S, Saruwatari A, Wang H Macromol Rapid Commun. 2024; 45(22):e2400419.

PMID: 39116444 PMC: 11583355. DOI: 10.1002/marc.202400419.

Sequence-encoded bioactive protein-multiblock polymer conjugates via quantitative one-pot iterative living polymerization.

Li Z, Song K, Chen Y, Huang Q, You L, Yu L Nat Commun. 2024; 15(1):6729.

PMID: 39112493 PMC: 11306232. DOI: 10.1038/s41467-024-51122-1.

Viscoplastic Modeling of Surface Relief Grating Growth on Isotropic and Preoriented Azopolymer Films.

Tverdokhleb N, Loebner S, Yadav B, Santer S, Saphiannikova M Polymers (Basel). 2023; 15(2).

PMID: 36679344 PMC: 9865333. DOI: 10.3390/polym15020463.

Recent Progress in Azopyridine-Containing Supramolecular Assembly: From Photoresponsive Liquid Crystals to Light-Driven Devices.

Ren H, Yang P, Yu H Molecules. 2022; 27(13).

PMID: 35807219 PMC: 9268027. DOI: 10.3390/molecules27133977.

References
1.
Bulmus V, Ding Z, Long C, Stayton P, Hoffman A . Site-specific polymer-streptavidin bioconjugate for pH-controlled binding and triggered release of biotin. Bioconjug Chem. 2000; 11(1):78-83. DOI: 10.1021/bc9901043. View
2.
Shimoboji T, Ding Z, Stayton P, Hoffman A . Mechanistic investigation of smart polymer-protein conjugates. Bioconjug Chem. 2001; 12(2):314-9. DOI: 10.1021/bc000107b. View
3.
Sandgren M, Shaw A, Ropp T, Wu S, Bott R, Cameron A . The X-ray crystal structure of the Trichoderma reesei family 12 endoglucanase 3, Cel12A, at 1.9 A resolution. J Mol Biol. 2001; 308(2):295-310. DOI: 10.1006/jmbi.2001.4583. View
4.
Ding Z, Fong R, Long C, Stayton P, Hoffman A . Size-dependent control of the binding of biotinylated proteins to streptavidin using a polymer shield. Nature. 2001; 411(6833):59-62. DOI: 10.1038/35075028. View
5.
Montagnoli G, Monti S, Nannicini L, Felicioli R . Azoaldolase photosensitivity. Photochem Photobiol. 1976; 23(1):29-32. DOI: 10.1111/j.1751-1097.1976.tb06766.x. View