DNA Microarray Analysis of Differentially Expressed Genes Responsive to Bisphenol A, an Alkylphenol Derivative, in an in Vitro Mouse Sertoli Cell Model
Overview
Affiliations
To identify genes elicited by bisphenol A (BPA) in Sertoli cells, we carried out a microarray analysis of TTE3 cells (a mouse Sertoli cell line) treated with BPA. BPA (100, 200 and 400 microM) induced cell death concentration-dependently, with levels being 25%, 33% and 96%, respectively. Of the 1,081 genes analyzed, 3 genes showed decreased levels of expression while the remaining 10 genes showed increased levels in the cells treated with a subtoxic dose of BPA (200 microM). The expressions of six genes were confirmed by the TaqMan assay. These findings suggest that DNA microarray analysis is a useful tool for investigating the molecular mechanisms of the toxic effects of BPA in testicular cells.
Neonatal bisphenol A exposure induces meiotic arrest and apoptosis of spermatogenic cells.
Xie M, Bu P, Li F, Lan S, Wu H, Yuan L Oncotarget. 2016; 7(9):10606-15.
PMID: 26863571 PMC: 4891144. DOI: 10.18632/oncotarget.7218.
DNA microarray-based gene expression profiling of estrogenic chemicals.
Kiyama R, Zhu Y Cell Mol Life Sci. 2014; 71(11):2065-82.
PMID: 24399289 PMC: 11113397. DOI: 10.1007/s00018-013-1544-5.
Bisphenol A effect on glutathione synthesis and recycling in testicular Sertoli cells.
Gualtieri A, Iwachow M, Venara M, Rey R, Schteingart H J Endocrinol Invest. 2010; 34(5):e102-9.
PMID: 20924222 DOI: 10.1007/BF03347468.
Meeker J, Ehrlich S, Toth T, Wright D, Calafat A, Trisini A Reprod Toxicol. 2010; 30(4):532-9.
PMID: 20656017 PMC: 2993767. DOI: 10.1016/j.reprotox.2010.07.005.
Meeker J, Calafat A, Hauser R Environ Sci Technol. 2009; 44(4):1458-63.
PMID: 20030380 PMC: 2823133. DOI: 10.1021/es9028292.