Differential Targeting of the Stress Mitogen-activated Protein Kinases to the C-Jun Dimerization Protein 2

Overview

Journal Biochem J

Specialty Biochemistry

Date 2002 Sep 13

PMID 12225289

Citations 17

Authors

Sigal Katz

Ami Aronheim

Affiliations

Soon will be listed here.

Abstract

The mitogen-activated kinases are structurally related proline-directed serine/threonine kinases that phosphorylate similar phosphoacceptor sites and yet, in vivo, they exhibit stringent substrate specificity. Specific targeting domains (kinase docking domains) facilitate kinase-substrate interaction and play a major role in substrate specificity determination. The c-Jun N-terminal kinase (JNK) consensus docking domain comprises of a KXXK/RXXXXLXL motif located in the delta-domain of the c-Jun N-terminal to the phosphoacceptor site. The c-Jun dimerization protein 2 is phosphorylated by JNK on Thr-148. Activating transcription factor 3 (ATF3) is a basic leucine zipper protein which is highly homologous to c-Jun dimerization protein 2 (JDP2), especially within the threonine/proline phosphoacceptor site, Thr-148. Nevertheless, ATF3 does not serve as a JNK substrate in vitro or in vivo. Using ATF3 and JDP2 protein chimaeras, we mapped the JNK-docking domain within JDP2. Although a JNK consensus putative docking site is located within the JDP2 leucine zipper motif, this domain does not function to recruit JNK to JDP2. A novel putative docking domain located C-terminally to the JDP2 phosphoacceptor site was identified. This domain, when fused to the ATF3 heterologous phosphoacceptor site, can direct its phosphorylation by JNK. In addition, although the novel JNK-docking domain was found to be necessary for p38 phosphorylation of JDP2 on Thr-148, it was not sufficient to confer JDP2 phosphorylation by the p38 kinase.

Citing Articles

Nuclear P38: Roles in Physiological and Pathological Processes and Regulation of Nuclear Translocation.

Maik-Rachline G, Lifshits L, Seger R Int J Mol Sci. 2020; 21(17).

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An overview of mammalian p38 mitogen-activated protein kinases, central regulators of cell stress and receptor signaling.

Han J, Wu J, Silke J F1000Res. 2020; 9.

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Mitochondrial oxidative stress-induced transcript variants of ATF3 mediate lipotoxic brain microvascular injury.

Nyunt T, Britton M, Wanichthanarak K, Budamagunta M, Voss J, Wilson D Free Radic Biol Med. 2019; 143:25-46.

PMID: 31356870 PMC: 6848793. DOI: 10.1016/j.freeradbiomed.2019.07.024.

JDP2 overexpression provokes cardiac dysfunction in mice.

Heger J, Bornbaum J, Wurfel A, Hill C, Brockmann N, Gaspar R Sci Rep. 2018; 8(1):7647.

PMID: 29769710 PMC: 5955919. DOI: 10.1038/s41598-018-26052-w.

Multiple functions of the histone chaperone Jun dimerization protein 2.

Tsai M, Wuputra K, Lin Y, Lin C, Yokoyama K Gene. 2016; 590(2):193-200.

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