Insufficient Expression of the Ilv-leu Operon Encoding Enzymes of Branched-chain Amino Acid Biosynthesis Limits Growth of a Bacillus Subtilis CcpA Mutant

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 2002 Aug 24

PMID 12193635

Citations 37

Authors

Holger Ludwig

Christoph Meinken

Anastasija Matin

Jorg Stulke

Affiliations

Soon will be listed here.

Abstract

Bacillus subtilis ccpA mutant strains exhibit two distinct phenotypes: they are defective in catabolite repression, and their growth on minimal media is strongly impaired. This growth defect is largely due to a lack of expression of the gltAB operon. However, growth is impaired even in the presence of glutamate. Here, we demonstrate that the ccpA mutant strain needs methionine and the branched-chain amino acids for optimal growth. The control of expression of the ilv-leu operon by CcpA provides a novel regulatory link between carbon and amino acid metabolism.

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