The Molecular Weights of Yeast Ribosomal Precursor RNAs

Overview

Journal Mol Biol Rep

Specialty Molecular Biology

Date 1975 Dec 1

PMID 1214784

Citations 11

Authors

R C Brand

R J Planta

Affiliations

Soon will be listed here.

Abstract

The molecular weights of the predominant rRNA precursors as well as those of 26-S and 17-S mature rRNA from Saccharomyces carlsbergensis were determined by polyacrylamide gel electrophoresis in the presence of formamide. Mature 26-S + 5.8-S rRNA was found to have a molecular weight of 1.24 X 10(6) while their immediate precursor, 29-S RNA, had a molecular weight of 1.52 X 10(6). Values of 0.70 X 10(6) and 0.82 X 10(6) were obtained for the molecular weights of mature 17-S rRNA and its 18-S precursor. Finally the 37-S precursor, common to both 29-S and 18-S RNA, was found to have a molecular weight of 2.80 X 10(6). Each precursor rRNA, therefore, contains extra sequences not found at the next stage of maturation.

Citing Articles

Synergistic defects in pre-rRNA processing from mutations in the U3-specific protein Rrp9 and U3 snoRNA.

Clerget G, Bourguignon-Igel V, Marmier-Gourrier N, Rolland N, Wacheul L, Manival X Nucleic Acids Res. 2020; 48(7):3848-3868.

PMID: 31996908 PMC: 7144924. DOI: 10.1093/nar/gkaa066.

The primary and secondary structure of yeast 26S rRNA.

Veldman G, Klootwijk J, de Regt V, Planta R, Branlant C, Krol A Nucleic Acids Res. 1981; 9(24):6935-52.

PMID: 7335496 PMC: 327652. DOI: 10.1093/nar/9.24.6935.

Some characteristics of processing sites in ribosomal precursor RNA of yeast.

Veldman G, Brand R, Klootwijk J, Planta R Nucleic Acids Res. 1980; 8(13):2907-20.

PMID: 6253896 PMC: 324134. DOI: 10.1093/nar/8.13.2907.

Heat-sensitive mutant strain of Neurospora crassa, 4M(t), conditionally defective in 25S ribosomal ribonucleic acid production.

Loo M, Schricker N, Russell P Mol Cell Biol. 1981; 1(3):199-207.

PMID: 6242828 PMC: 369663. DOI: 10.1128/mcb.1.3.199-207.1981.

Minimal methylation of yeast messenger RNA.

Mager W, Klootwijk J, Klein I Mol Biol Rep. 1976; 3(1):9-17.

PMID: 1004508 DOI: 10.1007/BF00357204.

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