Epigallocatechin Gallate, a Potential Immunomodulatory Agent of Tea Components, Diminishes Cigarette Smoke Condensate-induced Suppression of Anti-Legionella Pneumophila Activity and Cytokine Responses of Alveolar Macrophages

Overview

Journal Clin Diagn Lab Immunol

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 2002 Jul 3

PMID 12093687

Citations 7

Authors

Kazuto Matsunaga

Thomas W Klein

Herman Friedman

Yoshimasa Yamamoto

Affiliations

Soon will be listed here.

Abstract

Even though cigarette smoking has been shown to suppress immune responses in the lungs, little is known about the effect of cigarette smoke components on respiratory infections. In the present study, the effects of cigarette smoke condensate (CSC) on bacterial replication in alveolar macrophages and the immune responses of macrophages to infection were examined. Furthermore, a possible immunotherapeutic effect of epigallocatechin gallate (EGCg), a major form of tea catechins, on the CSC-induced suppression of antimicrobial activity and immune responses of alveolar macrophages was also determined. The treatment of murine alveolar macrophage cell line (MH-S) cells with CSC significantly enhanced the replication of Legionella pneumophila in macrophages and selectively down-regulated the production of interleukin-6 (IL-6) and tumor necrosis factor alpha (TNF-alpha) induced by bacterial infection. The treatment of macrophages with EGCg not only overcame the CSC-induced suppression of antimicrobial activity but also strengthened the resistance of macrophages to infection. EGCg also markedly up-regulated the CSC-suppressed IL-6 and TNF-alpha production by macrophages in response to infection. The results of exogenous TNF-alpha treatment and neutralization treatment with anti-TNF-alpha and anti-gamma-interferon (IFN-gamma) antibodies and the determination of IFN-gamma mRNA levels indicate that CSC-suppressed macrophages can be activated by EGCg to inhibit L. pneumophila growth by up-regulation of TNF-alpha and IFN-gamma production. Thus, this study revealed that CSC selectively alters the immune responses of macrophages to L. pneumophila infection and leads to an enhancement of bacterial replication in macrophages. In addition, the tea catechin EGCg can diminish such suppressive effects of CSC on alveolar macrophages.

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References

Ouyang Y, Virasch N, Hao P, Aubrey M, MUKERJEE N, Bierer B . Suppression of human IL-1beta, IL-2, IFN-gamma, and TNF-alpha production by cigarette smoke extracts. J Allergy Clin Immunol. 2000; 106(2):280-7. DOI: 10.1067/mai.2000.107751. View

McHugh S, Newton C, Yamamoto Y, Klein T, Friedman H . Tumor necrosis factor induces resistance of macrophages to Legionella pneumophila infection. Proc Soc Exp Biol Med. 2000; 224(3):191-6. DOI: 10.1046/j.1525-1373.2000.22420.x. View

Matsunaga K, Klein T, Friedman H, Yamamoto Y . Alveolar macrophage cell line MH-S is valuable as an in vitro model for Legionella pneumophila infection. Am J Respir Cell Mol Biol. 2001; 24(3):326-31. DOI: 10.1165/ajrcmb.24.3.4359. View

Matsunaga K, Klein T, Friedman H, Yamamoto Y . Legionella pneumophila replication in macrophages inhibited by selective immunomodulatory effects on cytokine formation by epigallocatechin gallate, a major form of tea catechins. Infect Immun. 2001; 69(6):3947-53. PMC: 98432. DOI: 10.1128/IAI.69.6.3947-3953.2001. View

Matsunaga K, Klein T, Friedman H, Yamamoto Y . Involvement of nicotinic acetylcholine receptors in suppression of antimicrobial activity and cytokine responses of alveolar macrophages to Legionella pneumophila infection by nicotine. J Immunol. 2001; 167(11):6518-24. DOI: 10.4049/jimmunol.167.11.6518. View

Shea J, Huber G, Holmes L, Homans A . The effect of experimental tobacco smoke inhalation on in vitro alveolar macrophage bactericidal function. J Lab Clin Med. 1978; 92(2):270-82. View

Horwitz M . Cell-mediated immunity in Legionnaires' disease. J Clin Invest. 1983; 71(6):1686-97. PMC: 370373. DOI: 10.1172/jci110923. View

Friedman H, Widen R, Klein T, Searls L, Cabrian K . Legionella pneumophila-induced blastogenesis of murine lymphoid cells in vitro. Infect Immun. 1984; 43(1):314-9. PMC: 263428. DOI: 10.1128/iai.43.1.314-319.1984. View

Read R . Presidential address. Systemic effects of smoking. Am J Surg. 1984; 148(6):706-11. DOI: 10.1016/0002-9610(84)90421-5. View

10.

Bhardwaj N, Nash T, Horwitz M . Interferon-gamma-activated human monocytes inhibit the intracellular multiplication of Legionella pneumophila. J Immunol. 1986; 137(8):2662-9. View

11.

Simonato L, Tomatis L . The International Agency for Research on Cancer experience in the evaluation of the carcinogenic risk to humans. Med Lav. 1986; 77(4):323-9. View

12.

Yamamoto Y, Klein T, Newton C, Widen R, Friedman H . Growth of Legionella pneumophila in thioglycolate-elicited peritoneal macrophages from A/J mice. Infect Immun. 1988; 56(2):370-5. PMC: 259290. DOI: 10.1128/iai.56.2.370-375.1988. View

13.

Nash T, Libby D, Horwitz M . IFN-gamma-activated human alveolar macrophages inhibit the intracellular multiplication of Legionella pneumophila. J Immunol. 1988; 140(11):3978-81. View

14.

Toda M, Okubo S, Ohnishi R, Shimamura T . [Antibacterial and bactericidal activities of Japanese green tea]. Nihon Saikingaku Zasshi. 1989; 44(4):669-72. DOI: 10.3412/jsb.44.669. View

15.

Flesch I, Kaufmann S . Stimulation of antibacterial macrophage activities by B-cell stimulatory factor 2 (interleukin-6). Infect Immun. 1990; 58(1):269-71. PMC: 258443. DOI: 10.1128/iai.58.1.269-271.1990. View

16.

Klein T, Yamamoto Y, Brown H, Friedman H . Interferon-gamma induced resistance to Legionella pneumophila in susceptible A/J mouse macrophages. J Leukoc Biol. 1991; 49(1):98-103. DOI: 10.1002/jlb.49.1.98. View

17.

Barral-Netto M, Barral A, Brownell C, Skeiky Y, Ellingsworth L, Twardzik D . Transforming growth factor-beta in leishmanial infection: a parasite escape mechanism. Science. 1992; 257(5069):545-8. DOI: 10.1126/science.1636092. View

18.

Liu Z, Simpson R, Cheers C . Recombinant interleukin-6 protects mice against experimental bacterial infection. Infect Immun. 1992; 60(10):4402-6. PMC: 257478. DOI: 10.1128/iai.60.10.4402-4406.1992. View

19.

Ho C, Chen Q, Shi H, Zhang K, Rosen R . Antioxidative effect of polyphenol extract prepared from various Chinese teas. Prev Med. 1992; 21(4):520-5. DOI: 10.1016/0091-7435(92)90059-q. View

20.

Soliman D, Twigg 3rd H . Cigarette smoking decreases bioactive interleukin-6 secretion by alveolar macrophages. Am J Physiol. 1992; 263(4 Pt 1):L471-8. DOI: 10.1152/ajplung.1992.263.4.L471. View