Modulation of Receptor Cycling by Neuron-enriched Endosomal Protein of 21 KD

Overview

Journal J Cell Biol

Specialty Cell Biology

Date 2002 Jun 19

PMID 12070131

Citations 48

Authors

Pascal Steiner

J-C Floyd Sarria

Liliane Glauser

Sarah Magnin

Stefan Catsicas

Harald Hirling

Affiliations

Soon will be listed here.

Abstract

Although correct cycling of neuronal membrane proteins is essential for neurite outgrowth and synaptic plasticity, neuron-specific proteins of the implicated endosomes have not been characterized. Here we show that a previously cloned, developmentally regulated, neuronal protein of unknown function binds to syntaxin 13. We propose to name this protein neuron-enriched endosomal protein of 21 kD (NEEP21), because it is colocalized with transferrin receptors, internalized transferrin (Tf), and Rab4. In PC12 cells, NEEP21 overexpression accelerates Tf internalization and recycling, whereas its down-regulation strongly delays Tf recycling. In primary neurons, NEEP21 is localized to the somatodendritic compartment, and, upon N-methyl-d-aspartate (NMDA) stimulation, the alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate receptor subunit GluR2 is internalized into NEEP21-positive endosomes. NEEP21 down-regulation retards recycling of GluR1 to the cell surface after NMDA stimulation of hippocampal neurons. In summary, NEEP21 is a neuronal protein that is localized to the early endosomal pathway and is necessary for correct receptor recycling in neurons.

Citing Articles

Neuron-Specific Gene Family Member 1 is a Potential New Therapeutic Target Associated with Immune Cell Infiltration for Breast Cancer.

Zhang H, Li Y, Wang R, Hu X, Wang Z Breast Cancer (Dove Med Press). 2024; 16:769-783.

PMID: 39564093 PMC: 11575459. DOI: 10.2147/BCTT.S483757.

O-GlcNAc forces an α-synuclein amyloid strain with notably diminished seeding and pathology.

Balana A, Mahul-Mellier A, Nguyen B, Horvath M, Javed A, Hard E Nat Chem Biol. 2024; 20(5):646-655.

PMID: 38347213 PMC: 11062923. DOI: 10.1038/s41589-024-01551-2.

Development and validation of an expanded antibody toolset that captures alpha-synuclein pathological diversity in Lewy body diseases.

Altay M, Kumar S, Burtscher J, Jagannath S, Strand C, Miki Y NPJ Parkinsons Dis. 2023; 9(1):161.

PMID: 38062007 PMC: 10703845. DOI: 10.1038/s41531-023-00604-y.

Nanoscale thermal control of a single living cell enabled by diamond heater-thermometer.

Romshin A, Zeeb V, Glushkov E, Radenovic A, Sinogeikin A, Vlasov I Sci Rep. 2023; 13(1):8546.

PMID: 37236978 PMC: 10220034. DOI: 10.1038/s41598-023-35141-4.

Aberrant NSG1 Expression Promotes Esophageal Squamous Cell Carcinoma Cell EMT by the Activation of ERK Signaling Pathway.

Lin X, Tu M, Zhang Y, Zhuang W, Cai L, Zhang L Dig Dis Sci. 2022; 68(5):1847-1857.

PMID: 36396779 DOI: 10.1007/s10620-022-07748-6.

References

Lippincott-Schwartz J, Yuan L, Tipper C, Amherdt M, Orci L, Klausner R . Brefeldin A's effects on endosomes, lysosomes, and the TGN suggest a general mechanism for regulating organelle structure and membrane traffic. Cell. 1991; 67(3):601-16. DOI: 10.1016/0092-8674(91)90534-6. View

Gorvel J, Chavrier P, Zerial M, Gruenberg J . rab5 controls early endosome fusion in vitro. Cell. 1991; 64(5):915-25. DOI: 10.1016/0092-8674(91)90316-q. View

van der Sluijs P, Hull M, Webster P, MALE P, Goud B, Mellman I . The small GTP-binding protein rab4 controls an early sorting event on the endocytic pathway. Cell. 1992; 70(5):729-40. DOI: 10.1016/0092-8674(92)90307-x. View

DAILEY M, Bridgman P . Vacuole dynamics in growth cones: correlated EM and video observations. J Neurosci. 1993; 13(8):3375-93. PMC: 6576535. View

Stenmark H, Parton R, LUTCKE A, Gruenberg J, Zerial M . Inhibition of rab5 GTPase activity stimulates membrane fusion in endocytosis. EMBO J. 1994; 13(6):1287-96. PMC: 394944. DOI: 10.1002/j.1460-2075.1994.tb06381.x. View

Rothman J . Mechanisms of intracellular protein transport. Nature. 1994; 372(6501):55-63. DOI: 10.1038/372055a0. View

Saberan-Djoneidi D, Picart R, Studler J, Tougard C, Glowinski J, Levi-Strauss M . A 19-kDa protein belonging to a new family is expressed in the Golgi apparatus of neural cells. J Biol Chem. 1995; 270(4):1888-93. DOI: 10.1074/jbc.270.4.1888. View

Gruenberg J, Maxfield F . Membrane transport in the endocytic pathway. Curr Opin Cell Biol. 1995; 7(4):552-63. DOI: 10.1016/0955-0674(95)80013-1. View

Xia Z, Dudek H, Miranti C, Greenberg M . Calcium influx via the NMDA receptor induces immediate early gene transcription by a MAP kinase/ERK-dependent mechanism. J Neurosci. 1996; 16(17):5425-36. PMC: 6578897. View

10.

Spiro D, Boll W, Kirchhausen T, Wessling-Resnick M . Wortmannin alters the transferrin receptor endocytic pathway in vivo and in vitro. Mol Biol Cell. 1996; 7(3):355-67. PMC: 275889. DOI: 10.1091/mbc.7.3.355. View

11.

Ullrich O, Reinsch S, Urbe S, Zerial M, Parton R . Rab11 regulates recycling through the pericentriolar recycling endosome. J Cell Biol. 1996; 135(4):913-24. PMC: 2133374. DOI: 10.1083/jcb.135.4.913. View

12.

Grimes M, Zhou J, Beattie E, Yuen E, Hall D, Valletta J . Endocytosis of activated TrkA: evidence that nerve growth factor induces formation of signaling endosomes. J Neurosci. 1996; 16(24):7950-64. PMC: 6579208. View

13.

Carlock L, Vo T, Lorincz M, Walker P, Bessert D, Wisniewski D . Variable subcellular localization of a neuron-specific protein during NTera 2 differentiation into post-mitotic human neurons. Brain Res Mol Brain Res. 1996; 42(2):202-12. DOI: 10.1016/s0169-328x(96)00115-5. View

14.

Wan Q, Xiong Z, Man H, Ackerley C, Braunton J, Lu W . Recruitment of functional GABA(A) receptors to postsynaptic domains by insulin. Nature. 1997; 388(6643):686-90. DOI: 10.1038/41792. View

15.

Malide D, Cushman S . Morphological effects of wortmannin on the endosomal system and GLUT4-containing compartments in rat adipose cells. J Cell Sci. 1998; 110 ( Pt 22):2795-806. DOI: 10.1242/jcs.110.22.2795. View

16.

McLauchlan H, Newell J, Morrice N, Osborne A, West M, Smythe E . A novel role for Rab5-GDI in ligand sequestration into clathrin-coated pits. Curr Biol. 1998; 8(1):34-45. DOI: 10.1016/s0960-9822(98)70018-1. View

17.

Saberan-Djoneidi D, Picart R, Escalier D, Gelman M, Barret A, Tougard C . A 21-kDa polypeptide belonging to a new family of proteins is expressed in the Golgi apparatus of neural and germ cells. J Biol Chem. 1998; 273(7):3909-14. DOI: 10.1074/jbc.273.7.3909. View

18.

Kobayashi T, Stang E, Fang K, de Moerloose P, Parton R, Gruenberg J . A lipid associated with the antiphospholipid syndrome regulates endosome structure and function. Nature. 1998; 392(6672):193-7. DOI: 10.1038/32440. View

19.

Tang B, Tan A, Lim L, Lee S, Low D, Hong W . Syntaxin 12, a member of the syntaxin family localized to the endosome. J Biol Chem. 1998; 273(12):6944-50. DOI: 10.1074/jbc.273.12.6944. View

20.

Prekeris R, Klumperman J, Chen Y, Scheller R . Syntaxin 13 mediates cycling of plasma membrane proteins via tubulovesicular recycling endosomes. J Cell Biol. 1998; 143(4):957-71. PMC: 2132958. DOI: 10.1083/jcb.143.4.957. View