Rab11 Regulates Recycling Through the Pericentriolar Recycling Endosome

Overview

Journal J Cell Biol

Specialty Cell Biology

Date 1996 Nov 1

PMID 8922376

Citations 620

Authors

O Ullrich

S Reinsch

S Urbe

M Zerial

R G Parton

Affiliations

Soon will be listed here.

Abstract

Small GTPases of the rab family are crucial elements of the machinery that controls membrane traffic. In the present study, we examined the distribution and function of rab11. Rab11 was shown by confocal immunofluorescence microscopy and EM to colocalize with internalized transferrin in the pericentriolar recycling compartment of CHO and BHK cells. Expression of rab11 mutants that are preferentially in the GTP- or GDP-bound state caused opposite effects on the distribution of transferrin-containing elements; rab11-GTP expression caused accumulation of labeled elements in the perinuclear area of the cell, whereas rab11-GDP caused a dispersion of the transferrin labeling. Functional studies showed that the early steps of uptake and recycling for transferrin were not affected by overexpression of rab11 proteins. However, recycling from the later recycling endosome was inhibited in cells overexpressing the rab11-GDP mutant. Rab5, which regulates early endocytic trafficking, acted before rab11 in the transferrin-recycling pathway as expression of rab5-GTP prevented transport to the rab11-positive recycling endosome. These results suggest a novel role for rab11 in controlling traffic through the recycling endosome.

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References

Futter C, Connolly C, Cutler D, Hopkins C . Newly synthesized transferrin receptors can be detected in the endosome before they appear on the cell surface. J Biol Chem. 1995; 270(18):10999-1003. DOI: 10.1074/jbc.270.18.10999. View

Simons K, Zerial M . Rab proteins and the road maps for intracellular transport. Neuron. 1993; 11(5):789-99. DOI: 10.1016/0896-6273(93)90109-5. View

Ghosh R, Maxfield F . Evidence for nonvectorial, retrograde transferrin trafficking in the early endosomes of HEp2 cells. J Cell Biol. 1995; 128(4):549-61. PMC: 2199884. DOI: 10.1083/jcb.128.4.549. View

Kelly R . Secretory granule and synaptic vesicle formation. Curr Opin Cell Biol. 1991; 3(4):654-60. DOI: 10.1016/0955-0674(91)90037-y. View

Martinez O, Schmidt A, Salamero J, Hoflack B, Roa M, Goud B . The small GTP-binding protein rab6 functions in intra-Golgi transport. J Cell Biol. 1994; 127(6 Pt 1):1575-88. PMC: 2120294. DOI: 10.1083/jcb.127.6.1575. View

McGraw T, Dunn K, Maxfield F . Isolation of a temperature-sensitive variant Chinese hamster ovary cell line with a morphologically altered endocytic recycling compartment. J Cell Physiol. 1993; 155(3):579-94. DOI: 10.1002/jcp.1041550316. View

Goud B, Zahraoui A, Tavitian A, Saraste J . Small GTP-binding protein associated with Golgi cisternae. Nature. 1990; 345(6275):553-6. DOI: 10.1038/345553a0. View

Stoorvogel W, Oorschot V, Geuze H . A novel class of clathrin-coated vesicles budding from endosomes. J Cell Biol. 1996; 132(1-2):21-33. PMC: 2120710. DOI: 10.1083/jcb.132.1.21. View

Chavrier P, Parton R, Hauri H, Simons K, Zerial M . Localization of low molecular weight GTP binding proteins to exocytic and endocytic compartments. Cell. 1990; 62(2):317-29. DOI: 10.1016/0092-8674(90)90369-p. View

10.

Goud B, McCaffrey M . Small GTP-binding proteins and their role in transport. Curr Opin Cell Biol. 1991; 3(4):626-33. DOI: 10.1016/0955-0674(91)90033-u. View

11.

Bucci C, Parton R, Mather I, Stunnenberg H, Simons K, Hoflack B . The small GTPase rab5 functions as a regulatory factor in the early endocytic pathway. Cell. 1992; 70(5):715-28. DOI: 10.1016/0092-8674(92)90306-w. View

12.

Bucci C, Wandinger-Ness A, LUTCKE A, Chiariello M, Bruni C, Zerial M . Rab5a is a common component of the apical and basolateral endocytic machinery in polarized epithelial cells. Proc Natl Acad Sci U S A. 1994; 91(11):5061-5. PMC: 43931. DOI: 10.1073/pnas.91.11.5061. View

13.

Ghosh R, Gelman D, Maxfield F . Quantification of low density lipoprotein and transferrin endocytic sorting HEp2 cells using confocal microscopy. J Cell Sci. 1994; 107 ( Pt 8):2177-89. DOI: 10.1242/jcs.107.8.2177. View

14.

LUTCKE A, Parton R, Murphy C, Olkkonen V, Dupree P, Valencia A . Cloning and subcellular localization of novel rab proteins reveals polarized and cell type-specific expression. J Cell Sci. 1994; 107 ( Pt 12):3437-48. DOI: 10.1242/jcs.107.12.3437. View

15.

Volknandt W, Pevsner J, Elferink L, Scheller R . Association of three small GTP-binding proteins with cholinergic synaptic vesicles. FEBS Lett. 1993; 317(1-2):53-6. DOI: 10.1016/0014-5793(93)81490-q. View

16.

Dunn K, McGraw T, Maxfield F . Iterative fractionation of recycling receptors from lysosomally destined ligands in an early sorting endosome. J Cell Biol. 1989; 109(6 Pt 2):3303-14. PMC: 2115921. DOI: 10.1083/jcb.109.6.3303. View

17.

Landt O, Grunert H, Hahn U . A general method for rapid site-directed mutagenesis using the polymerase chain reaction. Gene. 1990; 96(1):125-8. DOI: 10.1016/0378-1119(90)90351-q. View

18.

Connolly C, Futter C, Gibson A, Hopkins C, Cutler D . Transport into and out of the Golgi complex studied by transfecting cells with cDNAs encoding horseradish peroxidase. J Cell Biol. 1994; 127(3):641-52. PMC: 2120231. DOI: 10.1083/jcb.127.3.641. View

19.

Zerial M, Melancon P, Schneider C, Garoff H . The transmembrane segment of the human transferrin receptor functions as a signal peptide. EMBO J. 1986; 5(7):1543-50. PMC: 1166978. DOI: 10.1002/j.1460-2075.1986.tb04395.x. View

20.

Parton R, Dotti C, Bacallao R, Kurtz I, Simons K, Prydz K . pH-induced microtubule-dependent redistribution of late endosomes in neuronal and epithelial cells. J Cell Biol. 1991; 113(2):261-74. PMC: 2288934. DOI: 10.1083/jcb.113.2.261. View