Analysis of the NRT2 Nitrate Transporter Family in Arabidopsis. Structure and Gene Expression

Overview

Journal Plant Physiol

Specialty Physiology

Date 2002 Jun 18

PMID 12068127

Citations 94

Authors

Mathilde Orsel

Anne Krapp

Francoise Daniel-Vedele

Affiliations

Soon will be listed here.

Abstract

Nitrate is an essential element for plant growth, both as a primary nutrient in the nitrogen assimilation pathway and as an important signal for plant development. The uptake of nitrate from the soil and its translocation throughout the plant has been the subject of intensive physiological and molecular studies. Using a reverse genetic approach, the AtNRT2.1 gene has been shown to be involved in the inducible component of the high-affinity nitrate transport system in Arabidopsis. The Arabidopsis Genome Initiative has released nearly the whole genome sequence of Arabidopsis, allowing the identification of a small NRT2 multigene family in this species. Thus, we investigated the phylogenetic relationship between NRT2 proteins belonging to several kingdoms and compared the structure of the different members of the Arabidopsis family. We analyzed, by semiquantitative reverse transcriptase-polymerase chain reaction, the expression pattern of each gene depending on plant organ and development or nutritional status, and compared the relative level of each gene by real-time polymerase chain reaction. We also evaluated the significance of each paralog on the basis of the relative levels of gene expression. The results are discussed in relation with distinct roles for the individual members of the AtNRT2 family.

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References

Gaymard F, Pilot G, Lacombe B, Bouchez D, Bruneau D, Boucherez J . Identification and disruption of a plant shaker-like outward channel involved in K+ release into the xylem sap. Cell. 1998; 94(5):647-55. DOI: 10.1016/s0092-8674(00)81606-2. View

Wang R, Crawford N . Genetic identification of a gene involved in constitutive, high-affinity nitrate transport in higher plants. Proc Natl Acad Sci U S A. 1996; 93(17):9297-301. PMC: 38636. DOI: 10.1073/pnas.93.17.9297. View

Terryn N, Heijnen L, De Keyser A, Van Asseldonck M, De Clercq R, Verbakel H . Evidence for an ancient chromosomal duplication in Arabidopsis thaliana by sequencing and analyzing a 400-kb contig at the APETALA2 locus on chromosome 4. FEBS Lett. 1999; 445(2-3):237-45. DOI: 10.1016/s0014-5793(99)00097-6. View

Richmond T, Somerville C . The cellulose synthase superfamily. Plant Physiol. 2000; 124(2):495-8. PMC: 1539280. DOI: 10.1104/pp.124.2.495. View

Bevan M, Mayer K, White O, Eisen J, Preuss D, Bureau T . Sequence and analysis of the Arabidopsis genome. Curr Opin Plant Biol. 2001; 4(2):105-10. DOI: 10.1016/s1369-5266(00)00144-8. View

Pelaz S, Ditta G, Baumann E, Wisman E, Yanofsky M . B and C floral organ identity functions require SEPALLATA MADS-box genes. Nature. 2000; 405(6783):200-3. DOI: 10.1038/35012103. View

Quesada A, Krapp A, Trueman L, Daniel-Vedele F, Fernandez E, Forde B . PCR-identification of a Nicotiana plumbaginifolia cDNA homologous to the high-affinity nitrate transporters of the crnA family. Plant Mol Biol. 1997; 34(2):265-74. DOI: 10.1023/a:1005872816881. View

Takahashi H, Smith F, Hawkesford M, Saito K . The roles of three functional sulphate transporters involved in uptake and translocation of sulphate in Arabidopsis thaliana. Plant J. 2000; 23(2):171-82. DOI: 10.1046/j.1365-313x.2000.00768.x. View

Rossato L, Laine P, Ourry A . Nitrogen storage and remobilization in Brassica napus L. during the growth cycle: nitrogen fluxes within the plant and changes in soluble protein patterns. J Exp Bot. 2001; 52(361):1655-63. View

10.

Wang R, Liu D, Crawford N . The Arabidopsis CHL1 protein plays a major role in high-affinity nitrate uptake. Proc Natl Acad Sci U S A. 1998; 95(25):15134-9. PMC: 24588. DOI: 10.1073/pnas.95.25.15134. View

11.

Siddiqi M, Glass A, Ruth T, Rufty T . Studies of the Uptake of Nitrate in Barley: I. Kinetics of NO(3) Influx. Plant Physiol. 1990; 93(4):1426-32. PMC: 1062690. DOI: 10.1104/pp.93.4.1426. View

12.

Mayer K, Schuller C, Wambutt R, Murphy G, Volckaert G, Pohl T . Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana. Nature. 2000; 402(6763):769-77. DOI: 10.1038/47134. View

13.

Liljegren S, Ditta G, Eshed Y, Savidge B, Bowman J, Yanofsky M . SHATTERPROOF MADS-box genes control seed dispersal in Arabidopsis. Nature. 2000; 404(6779):766-70. DOI: 10.1038/35008089. View

14.

Lejay L, Tillard P, Lepetit M, Olive F, Filleur S, Daniel-Vedele F . Molecular and functional regulation of two NO3- uptake systems by N- and C-status of Arabidopsis plants. Plant J. 1999; 18(5):509-19. DOI: 10.1046/j.1365-313x.1999.00480.x. View

15.

Yokoyama R, Nishitani K . A comprehensive expression analysis of all members of a gene family encoding cell-wall enzymes allowed us to predict cis-regulatory regions involved in cell-wall construction in specific organs of Arabidopsis. Plant Cell Physiol. 2001; 42(10):1025-33. DOI: 10.1093/pcp/pce154. View

16.

Kronzucker H, Siddiqi M, Glass A . Kinetics of NO3- Influx in Spruce. Plant Physiol. 1995; 109(1):319-326. PMC: 157591. DOI: 10.1104/pp.109.1.319. View

17.

Orsel M, Filleur S, Fraisier V, Daniel-Vedele F . Nitrate transport in plants: which gene and which control?. J Exp Bot. 2002; 53(370):825-33. DOI: 10.1093/jexbot/53.370.825. View

18.

Zhou J, Fernandez E, Galvan A, Miller A . A high affinity nitrate transport system from Chlamydomonas requires two gene products. FEBS Lett. 2000; 466(2-3):225-7. DOI: 10.1016/s0014-5793(00)01085-1. View

19.

Fraisier V, Gojon A, Tillard P, Daniel-Vedele F . Constitutive expression of a putative high-affinity nitrate transporter in Nicotiana plumbaginifolia: evidence for post-transcriptional regulation by a reduced nitrogen source. Plant J. 2000; 23(4):489-96. DOI: 10.1046/j.1365-313x.2000.00813.x. View

20.

Cerezo M, Tillard P, Filleur S, Munos S, Daniel-Vedele F, Gojon A . Major alterations of the regulation of root NO(3)(-) uptake are associated with the mutation of Nrt2.1 and Nrt2.2 genes in Arabidopsis. Plant Physiol. 2001; 127(1):262-71. PMC: 117982. DOI: 10.1104/pp.127.1.262. View