Nitrate Transporter 2 () Family Genes, Identification, and Their Potential Functions in Abiotic Stress Tolerance

Overview

Journal Genes (Basel)

Publisher MDPI

Date 2023 Aug 26

PMID 37628616

Authors

Bingcan Lv

Yifan Li

Xiaoyu Wu

Chen Zhu

Yunyun Cao

Qiaohong Duan

Jiabao Huang

Affiliations

Soon will be listed here.

Abstract

Nitrate transporter 2 (NRT2) proteins play vital roles in both nitrate (NO) uptake and translocation as well as abiotic stress responses in plants. However, little is known about the gene family in . In this study, 14 were identified in the genome. The family members contain the PLN00028 and MATE_like superfamily domains. -element analysis indicated that regulatory elements related to stress responses are abundant in the promoter sequences of genes. expression was increased after drought stress, and and expression were significantly upregulated after salt stress. Furthermore, protein interaction predictions suggested that homologs of BrNRT2.3, BrNRT2.1, and BrNRT2.8 in may interact with the known stress-regulating proteins AtNRT1.1, AtNRT1.5, and AtNRT1.8. In conclusion, we suggest that , and have the greatest potential for inducing abiotic stress tolerance. Our findings will aid future studies of the biological functions of family genes.

Citing Articles

Genome-wide identification of high-affinity nitrate transporter 2 (NRT2) gene family under phytohormones and abiotic stresses in alfalfa (Medicago sativa).

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PMID: 39738449 PMC: 11685795. DOI: 10.1038/s41598-024-83438-9.

References

Kumar S, Stecher G, Li M, Knyaz C, Tamura K . MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms. Mol Biol Evol. 2018; 35(6):1547-1549. PMC: 5967553. DOI: 10.1093/molbev/msy096. View

Li J, Fu Y, Pike S, Bao J, Tian W, Zhang Y . The Arabidopsis nitrate transporter NRT1.8 functions in nitrate removal from the xylem sap and mediates cadmium tolerance. Plant Cell. 2010; 22(5):1633-46. PMC: 2899866. DOI: 10.1105/tpc.110.075242. View

Liu X, Zhu Y, Fang X, Ye J, Du W, Zhu Q . Ammonium aggravates salt stress in plants by entrapping them in a chloride over-accumulation state in an NRT1.1-dependent manner. Sci Total Environ. 2020; 746:141244. DOI: 10.1016/j.scitotenv.2020.141244. View

Feng H, Li B, Zhi Y, Chen J, Li R, Xia X . Overexpression of the nitrate transporter, OsNRT2.3b, improves rice phosphorus uptake and translocation. Plant Cell Rep. 2017; 36(8):1287-1296. DOI: 10.1007/s00299-017-2153-9. View

Chen Y, Yu P, Luo J, Jiang Y . Secreted protein prediction system combining CJ-SPHMM, TMHMM, and PSORT. Mamm Genome. 2004; 14(12):859-65. DOI: 10.1007/s00335-003-2296-6. View

Gu C, Song A, Zhang X, Wang H, Li T, Chen Y . Cloning of chrysanthemum high-affinity nitrate transporter family (CmNRT2) and characterization of CmNRT2.1. Sci Rep. 2016; 6:23462. PMC: 4804277. DOI: 10.1038/srep23462. View

Li G, Tillard P, Gojon A, Maurel C . Dual regulation of root hydraulic conductivity and plasma membrane aquaporins by plant nitrate accumulation and high-affinity nitrate transporter NRT2.1. Plant Cell Physiol. 2016; 57(4):733-42. DOI: 10.1093/pcp/pcw022. View

Zheng D, Han X, An Y, Guo H, Xia X, Yin W . The nitrate transporter NRT2.1 functions in the ethylene response to nitrate deficiency in Arabidopsis. Plant Cell Environ. 2013; 36(7):1328-37. DOI: 10.1111/pce.12062. View

Fan X, Tang Z, Tan Y, Zhang Y, Luo B, Yang M . Overexpression of a pH-sensitive nitrate transporter in rice increases crop yields. Proc Natl Acad Sci U S A. 2016; 113(26):7118-23. PMC: 4932942. DOI: 10.1073/pnas.1525184113. View

10.

Akbudak M, Filiz E, Cetin D . Genome-wide identification and characterization of high-affinity nitrate transporter 2 (NRT2) gene family in tomato (Solanum lycopersicum) and their transcriptional responses to drought and salinity stresses. J Plant Physiol. 2022; 272:153684. DOI: 10.1016/j.jplph.2022.153684. View

11.

Qiu Z, Guo J, Zhu A, Zhang L, Zhang M . Exogenous jasmonic acid can enhance tolerance of wheat seedlings to salt stress. Ecotoxicol Environ Saf. 2014; 104:202-8. DOI: 10.1016/j.ecoenv.2014.03.014. View

12.

Jeffares D, Penkett C, Bahler J . Rapidly regulated genes are intron poor. Trends Genet. 2008; 24(8):375-8. DOI: 10.1016/j.tig.2008.05.006. View

13.

Choi M, Kim E, Song J, Choi S, Cho S, Park C . Peptide transporter2 (PTR2) enhances water uptake during early seed germination in Arabidopsis thaliana. Plant Mol Biol. 2020; 102(6):615-624. PMC: 7062858. DOI: 10.1007/s11103-020-00967-3. View

14.

Dechorgnat J, Patrit O, Krapp A, Fagard M, Daniel-Vedele F . Characterization of the Nrt2.6 gene in Arabidopsis thaliana: a link with plant response to biotic and abiotic stress. PLoS One. 2012; 7(8):e42491. PMC: 3413667. DOI: 10.1371/journal.pone.0042491. View

15.

Brownlee A, Arst Jr H . Nitrate uptake in Aspergillus nidulans and involvement of the third gene of the nitrate assimilation gene cluster. J Bacteriol. 1983; 155(3):1138-46. PMC: 217809. DOI: 10.1128/jb.155.3.1138-1146.1983. View

16.

Tong J, Walk T, Han P, Chen L, Shen X, Li Y . Genome-wide identification and analysis of high-affinity nitrate transporter 2 (NRT2) family genes in rapeseed (Brassica napus L.) and their responses to various stresses. BMC Plant Biol. 2020; 20(1):464. PMC: 7547492. DOI: 10.1186/s12870-020-02648-1. View

17.

Cerezo M, Tillard P, Filleur S, Munos S, Daniel-Vedele F, Gojon A . Major alterations of the regulation of root NO(3)(-) uptake are associated with the mutation of Nrt2.1 and Nrt2.2 genes in Arabidopsis. Plant Physiol. 2001; 127(1):262-71. PMC: 117982. DOI: 10.1104/pp.127.1.262. View

18.

Sherman B, Hao M, Qiu J, Jiao X, Baseler M, Lane H . DAVID: a web server for functional enrichment analysis and functional annotation of gene lists (2021 update). Nucleic Acids Res. 2022; 50(W1):W216-W221. PMC: 9252805. DOI: 10.1093/nar/gkac194. View

19.

Verma S, Negi N, Pareek S, Mudgal G, Kumar D . Auxin response factors in plant adaptation to drought and salinity stress. Physiol Plant. 2022; 174(3):e13714. DOI: 10.1111/ppl.13714. View

20.

Liu J, Wu Y, Dong G, Zhu G, Zhou G . Progress of Research on the Physiology and Molecular Regulation of Sorghum Growth under Salt Stress by Gibberellin. Int J Mol Sci. 2023; 24(7). PMC: 10094886. DOI: 10.3390/ijms24076777. View