Initial Activation of Cyclin-B1-cdc2 Kinase Requires Phosphorylation of Cyclin B1

Overview

Journal EMBO Rep

Specialty Molecular Biology

Date 2002 May 30

PMID 12034754

Citations 12

Authors

Marion Peter

Christian LE Peuch

Jean-Claude Labbe

April N Meyer

Daniel J Donoghue

Marcel Doree

Affiliations

Soon will be listed here.

Abstract

At the G(2)/M transition of the cell cycle, the cdc25c phosphatase dephosphorylates inhibitory residues of cdc2, and cyclin-B-cdc2 kinase (MPF) is activated. Phosphorylation of cyclin B1 induces its nuclear accumulation, and, since cdc25c is also believed to accumulate and activate shortly before G(2)/M in the nucleus, it has been proposed that this induces cyclin-B1-cdc2 kinase activation. We demonstrate that cyclin B1 phosphorylation has another essential function in vivo: it is required for cdc25c and MPF activation, which does not require nuclear accumulation of cyclin B1, and occurs in the cytoplasm.

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