An Mt(+) Gamete-specific Nuclease That Targets Mt(-) Chloroplasts During Sexual Reproduction in C. Reinhardtii

Overview

Journal Genes Dev

Specialty Molecular Biology

Date 2002 May 10

PMID 12000794

Citations 30

Authors

Yoshiki Nishimura

Osami Misumi

Ko Kato

Noriko Inada

Tetsuya Higashiyama

Yu Momoyama

Tsuneyoshi Kuroiwa

Affiliations

Soon will be listed here.

Abstract

Although the active digestion of mating-type minus (mt-) chloroplast DNA (cpDNA) in young zygotes is considered to be the basis for the uniparental inheritance of cpDNA in Chlamydomonas reinhardtii, little is known about the underlying molecular mechanism. One model of active digestion proposes that nucleases are either synthesized or activated to digest mt- cpDNA. We used a native-PAGE/in gelo assay to investigate nuclease activities in chloroplasts from young zygotes, and identified a novel Ca(2+)-dependent nuclease activity. The timing of activation (approximately 60-90 min after mating) and the localization of the nuclease activity (in mt- chloroplasts) coincided with the active digestion of mt- cpDNA. Furthermore, the activity of the nuclease was coregulated with the maturation of mating-type plus (mt+) gametes, which would enable the efficient digestion of mt- cpDNA. Based on these observations, we propose that the nuclease (designated as Mt(+)-specific DNase, MDN) is a developmentally controlled nuclease that is activated in mt+ gametes and participates in the destruction of mt- cpDNA in young zygotes, thereby ensuring uniparental inheritance of chloroplast traits.

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