A DNA Vector-based RNAi Technology to Suppress Gene Expression in Mammalian Cells

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2002 Apr 18

PMID 11960009

Citations 349

Authors

Guangchao Sui

Christina Soohoo

El Bachir Affar

Frederique Gay

Yujiang Shi

William C Forrester

Yang Shi

Affiliations

Soon will be listed here.

Abstract

Double-stranded RNA-mediated interference (RNAi) has recently emerged as a powerful reverse genetic tool to silence gene expression in multiple organisms including plants, Caenorhabditis elegans, and Drosophila. The discovery that synthetic double-stranded, 21-nt small interfering RNA triggers gene-specific silencing in mammalian cells has further expanded the utility of RNAi into mammalian systems. Here we report a technology that allows synthesis of small interfering RNAs from DNA templates in vivo to efficiently inhibit endogenous gene expression. Significantly, we were able to use this approach to demonstrate, in multiple cell lines, robust inhibition of several endogenous genes of diverse functions. These findings highlight the general utility of this DNA vector-based RNAi technology in suppressing gene expression in mammalian cells.

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