The Cytolethal Distending Toxin of Haemophilus Ducreyi Inhibits Endothelial Cell Proliferation

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 2002 Apr 16

PMID 11953409

Citations 4

Authors

Liselott A Svensson

Petra Henning

Teresa Lagergard

Affiliations

Soon will be listed here.

Abstract

Haemophilus ducreyi, the etiologic agent of the sexually transmitted disease chancroid, produces a cytolethal distending toxin (HdCDT) that inhibits mammalian cell proliferation. We investigated the effects of HdCDT on normal human endothelial cells and on tubule formation in an in vitro model of angiogenesis. Endothelial cells were arrested in the G2 phase of the cell cycle, and tubule formation was inhibited in a dose-dependent manner. The antiproliferative activities of HdCDT on endothelial cells might contribute to the characteristic slow healing and persistence of chancroid ulcers.

Citing Articles

Impact of CDT Toxin on Human Diseases.

Fais T, Delmas J, Serres A, Bonnet R, Dalmasso G Toxins (Basel). 2016; 8(7).

PMID: 27429000 PMC: 4963852. DOI: 10.3390/toxins8070220.

The biology of the cytolethal distending toxins.

Guerra L, Cortes-Bratti X, Guidi R, Frisan T Toxins (Basel). 2011; 3(3):172-90.

PMID: 22069704 PMC: 3202825. DOI: 10.3390/toxins3030172.

Effector prediction in host-pathogen interaction based on a Markov model of a ubiquitous EPIYA motif.

Xu S, Zhang C, Miao Y, Gao J, Xu D BMC Genomics. 2010; 11 Suppl 3:S1.

PMID: 21143776 PMC: 2999339. DOI: 10.1186/1471-2164-11-S3-S1.

Cytolethal distending toxin from Shiga toxin-producing Escherichia coli O157 causes irreversible G2/M arrest, inhibition of proliferation, and death of human endothelial cells.

Bielaszewska M, Sinha B, Kuczius T, Karch H Infect Immun. 2004; 73(1):552-62.

PMID: 15618195 PMC: 538959. DOI: 10.1128/IAI.73.1.552-562.2005.

References

Lara-Tejero M, Galan J . A bacterial toxin that controls cell cycle progression as a deoxyribonuclease I-like protein. Science. 2000; 290(5490):354-7. DOI: 10.1126/science.290.5490.354. View

Deng K, Latimer J, Lewis D, Hansen E . Investigation of the interaction among the components of the cytolethal distending toxin of Haemophilus ducreyi. Biochem Biophys Res Commun. 2001; 285(3):609-15. DOI: 10.1006/bbrc.2001.5223. View

Frisk A, Lebens M, Johansson C, Ahmed H, Svensson L, AHLMAN K . The role of different protein components from the Haemophilus ducreyi cytolethal distending toxin in the generation of cell toxicity. Microb Pathog. 2001; 30(6):313-24. DOI: 10.1006/mpat.2000.0436. View

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Okuda J, Kurazono H, Takeda Y . Distribution of the cytolethal distending toxin A gene (cdtA) among species of Shigella and Vibrio, and cloning and sequencing of the cdt gene from Shigella dysenteriae. Microb Pathog. 1995; 18(3):167-72. DOI: 10.1016/s0882-4010(95)90022-5. View

Cope L, Lumbley S, Latimer J, STEVENS M, Johnson L, Purven M . A diffusible cytotoxin of Haemophilus ducreyi. Proc Natl Acad Sci U S A. 1997; 94(8):4056-61. PMC: 20567. DOI: 10.1073/pnas.94.8.4056. View

Young V, Knox K, Schauer D . Cytolethal distending toxin sequence and activity in the enterohepatic pathogen Helicobacter hepaticus. Infect Immun. 1999; 68(1):184-91. PMC: 97119. DOI: 10.1128/IAI.68.1.184-191.2000. View

Pickett C, Pesci E, Cottle D, Russell G, Erdem A, Zeytin H . Prevalence of cytolethal distending toxin production in Campylobacter jejuni and relatedness of Campylobacter sp. cdtB gene. Infect Immun. 1996; 64(6):2070-8. PMC: 174038. DOI: 10.1128/iai.64.6.2070-2078.1996. View

Sholley M, Ferguson G, Seibel H, MONTOUR J, Wilson J . Mechanisms of neovascularization. Vascular sprouting can occur without proliferation of endothelial cells. Lab Invest. 1984; 51(6):624-34. View

10.

Saiki K, Konishi K, Gomi T, Nishihara T, Yoshikawa M . Reconstitution and purification of cytolethal distending toxin of Actinobacillus actinomycetemcomitans. Microbiol Immunol. 2001; 45(6):497-506. DOI: 10.1111/j.1348-0421.2001.tb02650.x. View

11.

Pickett C, Whitehouse C . The cytolethal distending toxin family. Trends Microbiol. 1999; 7(7):292-7. DOI: 10.1016/s0966-842x(99)01537-1. View

12.

Elwell C, Dreyfus L . DNase I homologous residues in CdtB are critical for cytolethal distending toxin-mediated cell cycle arrest. Mol Microbiol. 2000; 37(4):952-63. DOI: 10.1046/j.1365-2958.2000.02070.x. View

13.

Sugai M, Kawamoto T, Peres S, Ueno Y, Komatsuzawa H, Fujiwara T . The cell cycle-specific growth-inhibitory factor produced by Actinobacillus actinomycetemcomitans is a cytolethal distending toxin. Infect Immun. 1998; 66(10):5008-19. PMC: 108622. DOI: 10.1128/IAI.66.10.5008-5019.1998. View

14.

Shenker B, Hoffmaster R, Zekavat A, Yamaguchi N, Lally E, Demuth D . Induction of apoptosis in human T cells by Actinobacillus actinomycetemcomitans cytolethal distending toxin is a consequence of G2 arrest of the cell cycle. J Immunol. 2001; 167(1):435-41. DOI: 10.4049/jimmunol.167.1.435. View

15.

SHELDON W, HEYMAN A . Studies on chancroid; observations on the histology with an evaluation of biopsy as a diagnostic procedure. Am J Pathol. 2010; 22:415-25. View

16.

Young R, Fortney K, Gelfanova V, Phillips C, Katz B, Hood A . Expression of cytolethal distending toxin and hemolysin is not required for pustule formation by Haemophilus ducreyi in human volunteers. Infect Immun. 2001; 69(3):1938-42. PMC: 98108. DOI: 10.1128/IAI.69.3.1938-1942.2001. View

17.

Denekamp J . Vascular endothelium as the vulnerable element in tumours. Acta Radiol Oncol. 1984; 23(4):217-25. DOI: 10.3109/02841868409136015. View

18.

Ahmed H, Svensson L, Cope L, Latimer J, Hansen E, AHLMAN K . Prevalence of cdtABC genes encoding cytolethal distending toxin among Haemophilus ducreyi and Actinobacillus actinomycetemcomitans strains. J Med Microbiol. 2001; 50(10):860-864. DOI: 10.1099/0022-1317-50-10-860. View

19.

Pickett C, Cottle D, Pesci E, Bikah G . Cloning, sequencing, and expression of the Escherichia coli cytolethal distending toxin genes. Infect Immun. 1994; 62(3):1046-51. PMC: 186222. DOI: 10.1128/iai.62.3.1046-1051.1994. View

20.

Mosmann T . Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods. 1983; 65(1-2):55-63. DOI: 10.1016/0022-1759(83)90303-4. View