Magnetite-alginate Beads for Purification of Some Starch Degrading Enzymes

Overview

Journal Mol Biotechnol

Publisher Springer

Specialties Biotechnology
Molecular Biology

Date 2002 Apr 9

PMID 11936253

Citations 1

Authors

Sunita Teotia

M N Gupta

Affiliations

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Abstract

Starch degrading enzymes, viz., beta-amylase, glucoamylase, and pullulanase, were purified using magnetite-alginate beads. In each case, the enzyme activity was eluted by using 1.0 M maltose. beta-Amylase (sweet potato), glucoamylase (Aspergillus niger), and pullulanase (Bacillus acidopullulyticus) from their crude preparations were purified 37-, 31-, and 49-fold with 86, 87, and 95% activity recovery, respectively. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis showed single band in each case.

Citing Articles

Magnetic techniques for the isolation and purification of proteins and peptides.

Safarik I, Safarikova M Biomagn Res Technol. 2004; 2(1):7.

PMID: 15566570 PMC: 544596. DOI: 10.1186/1477-044X-2-7.

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