Magnetic Techniques for the Isolation and Purification of Proteins and Peptides

Overview

Journal Biomagn Res Technol

Publisher Biomed Central

Specialty Biotechnology

Date 2004 Nov 30

PMID 15566570

Citations 63

Authors

Ivo Safarik

Mirka Safarikova

Affiliations

Soon will be listed here.

Abstract

Isolation and separation of specific molecules is used in almost all areas of biosciences and biotechnology. Diverse procedures can be used to achieve this goal. Recently, increased attention has been paid to the development and application of magnetic separation techniques, which employ small magnetic particles. The purpose of this review paper is to summarize various methodologies, strategies and materials which can be used for the isolation and purification of target proteins and peptides with the help of magnetic field. An extensive list of realised purification procedures documents the efficiency of magnetic separation techniques.

Citing Articles

Multiparameter screen optimizes immunoprecipitation.

Xie S, Saba L, Jiang H, Bringas O, Oghbaie M, Di Stefano L Biotechniques. 2024; 76(4):145-152.

PMID: 38425263 PMC: 11091867. DOI: 10.2144/btn-2023-0051.

Development of a rapid, efficient, and reusable magnetic bead-based immunocapture system for recombinant human procollagen type II isolation from yeast fermentation broth.

Lioi M, Tengattini S, Bagatin F, Galliani S, Daly S, Massolini G Anal Bioanal Chem. 2023; 415(16):3155-3166.

PMID: 37246979 PMC: 10287800. DOI: 10.1007/s00216-023-04752-1.

Recent Advances in Supramolecular Affinity Separations: Affinity Chromatography and Related Methods.

Woolfork A, Iftekhar S, Ovbude S, Suh K, Sharmeen S, Kyei I Adv Chromatogr. 2022; 58:1-74.

PMID: 36186535 PMC: 9520669. DOI: 10.1201/9781003223405-1.

Mag-spinner: a next-generation Facile, Affordable, Simple, and porTable (FAST) magnetic separation system.

Lee S, Jeong M, Lee S, Lee S, Choi J Nanoscale Adv. 2022; 4(3):792-800.

PMID: 36131828 PMC: 9419614. DOI: 10.1039/d1na00791b.

Progress in affinity ligand-functionalized bacterial magnetosome nanoparticles for bio-immunomagnetic separation of HBsAg protein.

Hatami Giklou Jajan L, Hosseini S, Abolhassani M, Ghorbani M PLoS One. 2022; 17(7):e0267206.

PMID: 35877673 PMC: 9312401. DOI: 10.1371/journal.pone.0267206.

References

Apoga D, Ek B, Tunlid A . Analysis of proteins in the extracellular matrix of the plant pathogenic fungus Bipolaris sorokiniana using 2-D gel electrophoresis and MS/MS. FEMS Microbiol Lett. 2001; 197(2):145-50. DOI: 10.1111/j.1574-6968.2001.tb10596.x. View

Himeji D, Horiuchi T, Tsukamoto H, Hayashi K, Watanabe T, Harada M . Characterization of caspase-8L: a novel isoform of caspase-8 that behaves as an inhibitor of the caspase cascade. Blood. 2002; 99(11):4070-8. DOI: 10.1182/blood.v99.11.4070. View

Fantappie M, Correa-Oliveira R, Caride E, Geraldo E, Agnew A, Rumjanek F . Comparison between site-specific DNA binding proteins of male and female Schistosoma mansoni. Comp Biochem Physiol B Biochem Mol Biol. 1999; 124(1):33-40. DOI: 10.1016/s0305-0491(99)00094-2. View

Tanaka T, Matsunaga T . Detection of HbA(1c) by boronate affinity immunoassay using bacterial magnetic particles. Biosens Bioelectron. 2001; 16(9-12):1089-94. DOI: 10.1016/s0956-5663(01)00187-7. View

Hollung K, Gabrielsen O, Jakobsen K . Enrichment of DNA-binding proteins from crude tissue for electrophoretic mobility shift assay using magnetic phospho cellulose particles. Nucleic Acids Res. 1994; 22(15):3261-2. PMC: 310312. DOI: 10.1093/nar/22.15.3261. View

Boffa L, Carpaneto E, Mariani M, Valentin E, Mendez E, Allfrey V . Recovery of transcriptionally active chromatin restriction fragments by binding to organomercurial-agarose magnetic beads. A rapid and sensitive method for monitoring changes in higher order chromatin structure during gene activation and repression. J Biol Chem. 1993; 268(31):23409-16. View

Bell P, Scheer U . Prenucleolar bodies contain coilin and are assembled in Xenopus egg extract depleted of specific nucleolar proteins and U3 RNA. J Cell Sci. 1997; 110 ( Pt 1):43-54. DOI: 10.1242/jcs.110.1.43. View

Heegaard N, Nilsson S, Guzman N . Affinity capillary electrophoresis: important application areas and some recent developments. J Chromatogr B Biomed Sci Appl. 1998; 715(1):29-54. DOI: 10.1016/s0378-4347(98)00258-8. View

Lee K, Park S, Mirkin C . Multicomponent magnetic nanorods for biomolecular separations. Angew Chem Int Ed Engl. 2004; 43(23):3048-50. DOI: 10.1002/anie.200454088. View

10.

Tyagi R, Gupta M . Immobilization of Aspergillus niger xylanase on magnetic latex beads. Biotechnol Appl Biochem. 1995; 21(2):217-22. DOI: 10.1111/j.1470-8744.1995.tb00332.x. View

11.

Safarik I, Safarikova M . Use of magnetic techniques for the isolation of cells. J Chromatogr B Biomed Sci Appl. 1999; 722(1-2):33-53. View

12.

Safarikova M, Roy I, Gupta M, Safarik I . Magnetic alginate microparticles for purification of alpha-amylases. J Biotechnol. 2003; 105(3):255-60. DOI: 10.1016/j.jbiotec.2003.07.002. View

13.

Hirschbein B, Whitesides G . Affinity separation of enzymes from mixtures containing suspended solids : Comparisons of magnetic and nonmagnetic techniques. Appl Biochem Biotechnol. 2013; 7(3):157-76. DOI: 10.1007/BF02798294. View

14.

Yaneva M, Tempst P . Affinity capture of specific DNA-binding proteins for mass spectrometric identification. Anal Chem. 2003; 75(23):6437-48. DOI: 10.1021/ac034698l. View

15.

Vinh J, Labas V, Kahn A, Phan D . Novel target sequences for Pax-6 in the brain-specific activating regions of the rat aldolase C gene. J Biol Chem. 2002; 277(49):47190-6. DOI: 10.1074/jbc.M209349200. View

16.

de Dios Alche J, Dickinson H . Affinity chromatographic purification of antibodies to a biotinylated fusion protein expressed in Escherichia coli. Protein Expr Purif. 1998; 12(1):138-43. DOI: 10.1006/prep.1997.0824. View

17.

Persaud D, Yousefi V, Haunerland N . Efficient isolation, purification, and characterization of the Helicoverpa zea VHDL receptor. Protein Expr Purif. 2004; 32(2):260-4. DOI: 10.1016/S1046-5928(03)00236-5. View

18.

Murphy M, Fuller S, Richardson P, Doyle S . An improved method for the in vitro evolution of aptamers and applications in protein detection and purification. Nucleic Acids Res. 2003; 31(18):e110. PMC: 203336. DOI: 10.1093/nar/gng110. View

19.

Griffin T, Mosbach K, Mosbach R . Magnetic biospecific affinity adsorbents for immunoglobulin and enzyme isolation. Appl Biochem Biotechnol. 2013; 6(4):283-92. DOI: 10.1007/BF02798279. View

20.

Schuster M, Wasserbauer E, Ortner C, Graumann K, Jungbauer A, Hammerschmid F . Short cut of protein purification by integration of cell-disrupture and affinity extraction. Bioseparation. 2000; 9(2):59-67. DOI: 10.1023/a:1008135913202. View