Mitochondrial Telomeres As Molecular Markers for Identification of the Opportunistic Yeast Pathogen Candida Parapsilosis

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 2002 Mar 30

PMID 11923346

Citations 19

Authors

Jozef Nosek

lubomir Tomaska

Adriana Rycovska

Hiroshi Fukuhara

Affiliations

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Abstract

Recent studies have demonstrated that a large number of organisms carry linear mitochondrial DNA molecules possessing specialized telomeric structures at their ends. Based on this specific structural feature of linear mitochondrial genomes, we have developed an approach for identification of the opportunistic yeast pathogen Candida parapsilosis. The strategy for identification of C. parapsilosis strains is based on PCR amplification of specific DNA sequences derived from the mitochondrial telomere region. This assay is complemented by immunodetection of a protein component of mitochondrial telomeres. The results demonstrate that mitochondrial telomeres represent specific molecular markers with potential applications in yeast diagnostics and taxonomy.

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References

Lott T, Kuykendall R, Welbel S, Pramanik A, Lasker B . Genomic heterogeneity in the yeast Candida parapsilosis. Curr Genet. 1993; 23(5-6):463-7. DOI: 10.1007/BF00312635. View

Fukuhara H, Sor F, Drissi R, Dinouel N, Miyakawa I, Rousset S . Linear mitochondrial DNAs of yeasts: frequency of occurrence and general features. Mol Cell Biol. 1993; 13(4):2309-14. PMC: 359551. DOI: 10.1128/mcb.13.4.2309-2314.1993. View

Branchini M, Pfaller M, Frempong T, ISENBERG H . Genotypic variation and slime production among blood and catheter isolates of Candida parapsilosis. J Clin Microbiol. 1994; 32(2):452-6. PMC: 263052. DOI: 10.1128/jcm.32.2.452-456.1994. View

Nosek J, Fukuhara H . NADH dehydrogenase subunit genes in the mitochondrial DNA of yeasts. J Bacteriol. 1994; 176(18):5622-30. PMC: 196764. DOI: 10.1128/jb.176.18.5622-5630.1994. View

Santos M, Cox A, Luz J, Keith G, Tuite M . Transfer RNA profiling: a new method for the identification of pathogenic Candida species. Yeast. 1994; 10(5):625-36. DOI: 10.1002/yea.320100507. View

James S, Collins M, Roberts I . The genetic relationship of Lodderomyces elongisporus to other ascomycete yeast species as revealed by small-subunit rRNA gene sequences. Lett Appl Microbiol. 1994; 19(5):308-11. DOI: 10.1111/j.1472-765x.1994.tb00462.x. View

Horvath A, Riezman H . Rapid protein extraction from Saccharomyces cerevisiae. Yeast. 1994; 10(10):1305-10. DOI: 10.1002/yea.320101007. View

Cassone A, De Bernardis F, Pontieri E, Carruba G, Girmenia C, Martino P . Biotype diversity of Candida parapsilosis and its relationship to the clinical source and experimental pathogenicity. J Infect Dis. 1995; 171(4):967-75. DOI: 10.1093/infdis/171.4.967. View

Nosek J, Dinouel N, Kovac L, Fukuhara H . Linear mitochondrial DNAs from yeasts: telomeres with large tandem repetitions. Mol Gen Genet. 1995; 247(1):61-72. DOI: 10.1007/BF00425822. View

10.

Pfaller M, Messer S, Hollis R . Variations in DNA subtype, antifungal susceptibility, and slime production among clinical isolates of Candida parapsilosis. Diagn Microbiol Infect Dis. 1995; 21(1):9-14. DOI: 10.1016/0732-8893(94)00114-c. View

11.

Lin D, Wu L, Rinaldi M, Lehmann P . Three distinct genotypes within Candida parapsilosis from clinical sources. J Clin Microbiol. 1995; 33(7):1815-21. PMC: 228276. DOI: 10.1128/jcm.33.7.1815-1821.1995. View

12.

Haynes K, Westerneng T . Rapid identification of Candida albicans, C. glabrata, C. parapsilosis and C. krusei by species-specific PCR of large subunit ribosomal DNA. J Med Microbiol. 1996; 44(5):390-6. DOI: 10.1099/00222615-44-5-390. View

13.

Sullivan D, Henman M, Moran G, ONeill L, Bennett D, Shanley D . Molecular genetic approaches to identification, epidemiology and taxonomy of non-albicans Candida species. J Med Microbiol. 1996; 44(6):399-408. DOI: 10.1099/00222615-44-6-399. View

14.

Pontieri E, Gregori L, Gennarelli M, Ceddia T, Novelli G, Dallapiccola B . Correlation of SfiI macrorestriction endonuclease fingerprint analysis of Candida parapsilosis isolates with source of isolation. J Med Microbiol. 1996; 45(3):173-8. DOI: 10.1099/00222615-45-3-173. View

15.

Girmenia C, Martino P, De Bernardis F, Gentile G, Boccanera M, Monaco M . Rising incidence of Candida parapsilosis fungemia in patients with hematologic malignancies: clinical aspects, predisposing factors, and differential pathogenicity of the causative strains. Clin Infect Dis. 1996; 23(3):506-14. DOI: 10.1093/clinids/23.3.506. View

16.

Tomaska L, Nosek J, Fukuhara H . Identification of a putative mitochondrial telomere-binding protein of the yeast Candida parapsilosis. J Biol Chem. 1997; 272(5):3049-56. DOI: 10.1074/jbc.272.5.3049. View

17.

Roy B, Meyer S . Confirmation of the distinct genotype groups within the form species Candida parapsilosis. J Clin Microbiol. 1998; 36(1):216-8. PMC: 124837. DOI: 10.1128/JCM.36.1.216-218.1998. View

18.

Nosek J, Tomaska L, Fukuhara H, Suyama Y, Kovac L . Linear mitochondrial genomes: 30 years down the line. Trends Genet. 1998; 14(5):184-8. DOI: 10.1016/s0168-9525(98)01443-7. View

19.

Mannarelli B, Kurtzman C . Rapid identification of Candida albicans and other human pathogenic yeasts by using short oligonucleotides in a PCR. J Clin Microbiol. 1998; 36(6):1634-41. PMC: 104892. DOI: 10.1128/JCM.36.6.1634-1641.1998. View

20.

Shukla G, Nene V . Telomeric features of Theileria parva mitochondrial DNA derived from cycle sequence data of total genomic DNA. Mol Biochem Parasitol. 1998; 95(1):159-63. DOI: 10.1016/s0166-6851(98)00098-x. View