Heat Shock Cognate Protein 70 is Involved in Rotavirus Cell Entry

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 2002 Mar 22

PMID 11907249

Citations 72

Authors

Carlos A Guerrero

Daniela Bouyssounade

Selene Zarate

Pavel Isa

Tomas Lopez

Rafaela Espinosa

Pedro Romero

Ernesto Mendez

Susana Lopez

Carlos F Arias

Affiliations

Soon will be listed here.

Abstract

In this work, we have identified the heat shock cognate protein (hsc70) as a receptor candidate for rotaviruses. hsc70 was shown to be present on the surface of MA104 cells, and antibodies to this protein blocked rotavirus infectivity, while not affecting the infectivity of reovirus and poliovirus. Preincubation of the hsc70 protein with the viruses also inhibited their infectivity. Triple-layered particles (mature virions), but not double-layered particles, bound hsc70 in a solid-phase assay, and this interaction was blocked by monoclonal antibodies to the virus surface proteins VP4 and VP7. Rotaviruses were shown to interact with hsc70 at a postattachment step, since antibodies to hsc70 and the protein itself did not inhibit the virus attachment to cells. We propose that the functional rotavirus receptor is a complex of several cell surface molecules that include, among others, hsc70.

Citing Articles

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