Osmoregulation of Dimer Resolution at the Plasmid PJHCMW1 Mwr Locus by Escherichia Coli XerCD Recombination

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 2002 Mar 2

PMID 11872712

Citations 8

Authors

Huong Pham

Ken J Dery

David J Sherratt

Marcelo E Tolmasky

Affiliations

Soon will be listed here.

Abstract

Xer-mediated dimer resolution at the mwr site of plasmid pJHCMW1 is osmoregulated in Escherichia coli. Whereas under low-salt conditions, the site-specific recombination reaction is efficient, under high-salt conditions, it proceeds inefficiently. Regulation of dimer resolution is independent of H-NS and is mediated by changes in osmolarity rather than ionic effects. The low level of recombination at high salt concentrations can be overcome by high levels of PepA or by mutating the ARG box to a sequence closer to the E. coli ARG box consensus. The central region of the mwr core recombination site plays a role in regulation of site-specific recombination by the osmotic pressure of the medium.

Citing Articles

Functional Analysis of the XerC and XerD Site-Specific Recombinases: Potential Role in Dissemination of Resistance Genes.

Lin D, Traglia G, Baker R, Sherratt D, Ramirez M, Tolmasky M Antibiotics (Basel). 2020; 9(7).

PMID: 32668667 PMC: 7399989. DOI: 10.3390/antibiotics9070405.

Small Plasmids: Neglected Contributors to Antibiotic Resistance.

Ramirez M, Iriarte A, Reyes-Lamothe R, Sherratt D, Tolmasky M Front Microbiol. 2019; 10:2182.

PMID: 31616398 PMC: 6764390. DOI: 10.3389/fmicb.2019.02182.

Plasmid-Mediated Antibiotic Resistance and Virulence in Gram-negatives: the Paradigm.

Ramirez M, Traglia G, Lin D, Tran T, Tolmasky M Microbiol Spectr. 2015; 2(5):1-15.

PMID: 25705573 PMC: 4335354. DOI: 10.1128/microbiolspec.PLAS-0016-2013.

Small plasmids harboring qnrB19: a model for plasmid evolution mediated by site-specific recombination at oriT and Xer sites.

Tran T, Andres P, Petroni A, Soler-Bistue A, Albornoz E, Zorreguieta A Antimicrob Agents Chemother. 2012; 56(4):1821-7.

PMID: 22290975 PMC: 3318318. DOI: 10.1128/AAC.06036-11.

fpr, a deficient Xer recombination site from a Salmonella plasmid, fails to confer stability by dimer resolution: comparative studies with the pJHCMW1 mwr site.

Tran T, Sherratt D, Tolmasky M J Bacteriol. 2009; 192(3):883-7.

PMID: 19966005 PMC: 2812456. DOI: 10.1128/JB.01082-09.

References

Tolmasky M, Colloms S, Blakely G, Sherratt D . Stability by multimer resolution of pJHCMW1 is due to the Tn1331 resolvase and not to the Escherichia coli Xer system. Microbiology (Reading). 2000; 146 ( Pt 3):581-589. DOI: 10.1099/00221287-146-3-581. View

Wood J . Osmosensing by bacteria: signals and membrane-based sensors. Microbiol Mol Biol Rev. 1999; 63(1):230-62. PMC: 98963. DOI: 10.1128/MMBR.63.1.230-262.1999. View

Nieto J, Madrid C, Prenafeta A, Miquelay E, Balsalobre C, Carrascal M . Expression of the hemolysin operon in Escherichia coli is modulated by a nucleoid-protein complex that includes the proteins Hha and H-NS. Mol Gen Genet. 2000; 263(2):349-58. DOI: 10.1007/s004380051178. View

Arciszewska L, Baker R, Hallet B, Sherratt D . Coordinated control of XerC and XerD catalytic activities during Holliday junction resolution. J Mol Biol. 2000; 299(2):391-403. DOI: 10.1006/jmbi.2000.3762. View

Barre F, Aroyo M, Colloms S, Helfrich A, Cornet F, Sherratt D . FtsK functions in the processing of a Holliday junction intermediate during bacterial chromosome segregation. Genes Dev. 2000; 14(23):2976-88. PMC: 317095. DOI: 10.1101/gad.188700. View

Barre F, Soballe B, Michel B, Aroyo M, Robertson M, Sherratt D . Circles: the replication-recombination-chromosome segregation connection. Proc Natl Acad Sci U S A. 2001; 98(15):8189-95. PMC: 37420. DOI: 10.1073/pnas.111008998. View

Cohen S, Chang A, Hsu L . Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA. Proc Natl Acad Sci U S A. 1972; 69(8):2110-4. PMC: 426879. DOI: 10.1073/pnas.69.8.2110. View

Summers D, Sherratt D . Multimerization of high copy number plasmids causes instability: CoIE1 encodes a determinant essential for plasmid monomerization and stability. Cell. 1984; 36(4):1097-103. DOI: 10.1016/0092-8674(84)90060-6. View

Tolmasky M, Roberts M, Woloj M, Crosa J . Molecular cloning of amikacin resistance determinants from a Klebsiella pneumoniae plasmid. Antimicrob Agents Chemother. 1986; 30(2):315-20. PMC: 180541. DOI: 10.1128/AAC.30.2.315. View

10.

Higgins C, Dorman C, Stirling D, Waddell L, Booth I, May G . A physiological role for DNA supercoiling in the osmotic regulation of gene expression in S. typhimurium and E. coli. Cell. 1988; 52(4):569-84. DOI: 10.1016/0092-8674(88)90470-9. View

11.

Tolmasky M, Crosa J . Tn1331, a novel multiresistance transposon encoding resistance to amikacin and ampicillin in Klebsiella pneumoniae. Antimicrob Agents Chemother. 1987; 31(12):1955-60. PMC: 175834. DOI: 10.1128/AAC.31.12.1955. View

12.

Summers D, Sherratt D . Resolution of ColE1 dimers requires a DNA sequence implicated in the three-dimensional organization of the cer site. EMBO J. 1988; 7(3):851-8. PMC: 454402. DOI: 10.1002/j.1460-2075.1988.tb02884.x. View

13.

Stirling C, Szatmari G, Stewart G, Smith M, Sherratt D . The arginine repressor is essential for plasmid-stabilizing site-specific recombination at the ColE1 cer locus. EMBO J. 1988; 7(13):4389-95. PMC: 455177. DOI: 10.1002/j.1460-2075.1988.tb03338.x. View

14.

Summers D . Derivatives of ColE1 cer show altered topological specificity in site-specific recombination. EMBO J. 1989; 8(1):309-15. PMC: 400805. DOI: 10.1002/j.1460-2075.1989.tb03378.x. View

15.

Stirling C, Colloms S, Collins J, Szatmari G, Sherratt D . xerB, an Escherichia coli gene required for plasmid ColE1 site-specific recombination, is identical to pepA, encoding aminopeptidase A, a protein with substantial similarity to bovine lens leucine aminopeptidase. EMBO J. 1989; 8(5):1623-7. PMC: 400995. DOI: 10.1002/j.1460-2075.1989.tb03547.x. View

16.

Colloms S, Sykora P, Szatmari G, Sherratt D . Recombination at ColE1 cer requires the Escherichia coli xerC gene product, a member of the lambda integrase family of site-specific recombinases. J Bacteriol. 1990; 172(12):6973-80. PMC: 210817. DOI: 10.1128/jb.172.12.6973-6980.1990. View

17.

Clerget M . Site-specific recombination promoted by a short DNA segment of plasmid R1 and by a homologous segment in the terminus region of the Escherichia coli chromosome. New Biol. 1991; 3(8):780-8. View

18.

Blakely G, Colloms S, May G, Burke M, Sherratt D . Escherichia coli XerC recombinase is required for chromosomal segregation at cell division. New Biol. 1991; 3(8):789-98. View

19.

KUEMPEL P, Henson J, Dircks L, Tecklenburg M, Lim D . dif, a recA-independent recombination site in the terminus region of the chromosome of Escherichia coli. New Biol. 1991; 3(8):799-811. View

20.

Cayley S, Lewis B, Guttman H, Record Jr M . Characterization of the cytoplasm of Escherichia coli K-12 as a function of external osmolarity. Implications for protein-DNA interactions in vivo. J Mol Biol. 1991; 222(2):281-300. DOI: 10.1016/0022-2836(91)90212-o. View