Characterization of a New Extended-spectrum Beta-lactamase (TEM-87) Isolated in Proteus Mirabilis During an Italian Survey

Overview

Journal Antimicrob Agents Chemother

Publisher American Society for Microbiology

Specialty Pharmacology

Date 2002 Feb 19

PMID 11850290

Citations 9

Authors

Mariagrazia Perilli

Bernardetta Segatore

Maria Rosaria De Massis

Nicola Franceschini

Ciro Bianchi

Gian Maria Rossolini

Gianfranco Amicosante

Affiliations

Soon will be listed here.

Abstract

A new natural TEM derivative, named TEM-87, was identified in a Proteus mirabilis isolate from an Italian hospital. Compared to TEM-1, TEM-87 contains the following mutations: E104K, R164C, and M182T. Kinetic analysis of TEM-87 revealed extended-spectrum activity against oxyimino cephalosporins (preferentially ceftazidime) and aztreonam. Expression of blaTEM-87 in Escherichia coli decreased the host susceptibility to these drugs.

Citing Articles

TEM-184, a Novel TEM-Derived Extended-Spectrum β-Lactamase with Enhanced Activity against Aztreonam.

Piccirilli A, Perilli M, Amicosante G, Conte V, Tascini C, Rossolini G Antimicrob Agents Chemother. 2018; 62(9).

PMID: 29941651 PMC: 6125571. DOI: 10.1128/AAC.00688-18.

TEM-187, a new extended-spectrum β-lactamase with weak activity in a Proteus mirabilis clinical strain.

Corvec S, Beyrouthy R, Cremet L, Aubin G, Robin F, Bonnet R Antimicrob Agents Chemother. 2013; 57(5):2410-2.

PMID: 23478954 PMC: 3632918. DOI: 10.1128/AAC.01761-12.

Multidrug-resistant Proteus mirabilis bloodstream infections: risk factors and outcomes.

Tumbarello M, Trecarichi E, Fiori B, Losito A, DInzeo T, Campana L Antimicrob Agents Chemother. 2012; 56(6):3224-31.

PMID: 22450979 PMC: 3370815. DOI: 10.1128/AAC.05966-11.

New TEM-derived extended-spectrum beta-lactamase and its genomic context in plasmids from Salmonella enterica serovar derby isolates from Uruguay.

Vignoli R, Cordeiro N, Garcia V, Mota M, Betancor L, Power P Antimicrob Agents Chemother. 2006; 50(2):781-4.

PMID: 16436745 PMC: 1366896. DOI: 10.1128/AAC.50.2.781-784.2006.

Biochemical characterization of laboratory mutants of extended-spectrum beta-lactamase TEM-60.

Caporale B, Franceschini N, Perilli M, Segatore B, Rossolini G, Amicosante G Antimicrob Agents Chemother. 2004; 48(9):3579-82.

PMID: 15328132 PMC: 514782. DOI: 10.1128/AAC.48.9.3579-3582.2004.

References

Bonnet R, De Champs C, Sirot D, Chanal C, Labia R, Sirot J . Diversity of TEM mutants in Proteus mirabilis. Antimicrob Agents Chemother. 1999; 43(11):2671-7. PMC: 89541. DOI: 10.1128/AAC.43.11.2671. View

Petrosino J, Cantu 3rd C, Palzkill T . beta-Lactamases: protein evolution in real time. Trends Microbiol. 1998; 6(8):323-7. DOI: 10.1016/s0966-842x(98)01317-1. View

Sutcliffe J . Nucleotide sequence of the ampicillin resistance gene of Escherichia coli plasmid pBR322. Proc Natl Acad Sci U S A. 1978; 75(8):3737-41. PMC: 392861. DOI: 10.1073/pnas.75.8.3737. View

Knothe H, Shah P, Krcmery V, Antal M, Mitsuhashi S . Transferable resistance to cefotaxime, cefoxitin, cefamandole and cefuroxime in clinical isolates of Klebsiella pneumoniae and Serratia marcescens. Infection. 1983; 11(6):315-7. DOI: 10.1007/BF01641355. View

De Meester F, Joris B, Reckinger G, Frere J, WALEY S . Automated analysis of enzyme inactivation phenomena. Application to beta-lactamases and DD-peptidases. Biochem Pharmacol. 1987; 36(14):2393-403. DOI: 10.1016/0006-2952(87)90609-5. View

Barthelemy M, Peduzzi J, Labia R . Complete amino acid sequence of p453-plasmid-mediated PIT-2 beta-lactamase (SHV-1). Biochem J. 1988; 251(1):73-9. PMC: 1148965. DOI: 10.1042/bj2510073. View

Sirot J, Chanal C, Petit A, Sirot D, Labia R, Gerbaud G . Klebsiella pneumoniae and other Enterobacteriaceae producing novel plasmid-mediated beta-lactamases markedly active against third-generation cephalosporins: epidemiologic studies. Rev Infect Dis. 1988; 10(4):850-9. DOI: 10.1093/clinids/10.4.850. View

Jarlier V, Nicolas M, Fournier G, Philippon A . Extended broad-spectrum beta-lactamases conferring transferable resistance to newer beta-lactam agents in Enterobacteriaceae: hospital prevalence and susceptibility patterns. Rev Infect Dis. 1988; 10(4):867-78. DOI: 10.1093/clinids/10.4.867. View

Mabilat C, Goussard S, Sougakoff W, Spencer R, Courvalin P . Direct sequencing of the amplified structural gene and promoter for the extended-broad-spectrum beta-lactamase TEM-9 (RHH-1) of Klebsiella pneumoniae. Plasmid. 1990; 23(1):27-34. DOI: 10.1016/0147-619x(90)90041-a. View

10.

Jacoby G, Medeiros A . More extended-spectrum beta-lactamases. Antimicrob Agents Chemother. 1991; 35(9):1697-704. PMC: 245253. DOI: 10.1128/AAC.35.9.1697. View

11.

Bradford P, CHERUBIN C, Idemyor V, Rasmussen B, Bush K . Multiply resistant Klebsiella pneumoniae strains from two Chicago hospitals: identification of the extended-spectrum TEM-12 and TEM-10 ceftazidime-hydrolyzing beta-lactamases in a single isolate. Antimicrob Agents Chemother. 1994; 38(4):761-6. PMC: 284539. DOI: 10.1128/AAC.38.4.761. View

12.

Knox J . Extended-spectrum and inhibitor-resistant TEM-type beta-lactamases: mutations, specificity, and three-dimensional structure. Antimicrob Agents Chemother. 1995; 39(12):2593-601. PMC: 162995. DOI: 10.1128/AAC.39.12.2593. View

13.

Bradford P, Jacobus N, Bhachech N, Bush K . TEM-28 from an Escherichia coli clinical isolate is a member of the His-164 family of TEM-1 extended-spectrum beta-lactamases. Antimicrob Agents Chemother. 1996; 40(1):260-2. PMC: 163097. DOI: 10.1128/AAC.40.1.260. View

14.

Perilli M, Felici A, Franceschini N, DE Santis A, Pagani L, Luzzaro F . Characterization of a new TEM-derived beta-lactamase produced in a Serratia marcescens strain. Antimicrob Agents Chemother. 1997; 41(11):2374-82. PMC: 164131. DOI: 10.1128/AAC.41.11.2374. View

15.

Jacoby G . Extended-spectrum beta-lactamases and other enzymes providing resistance to oxyimino-beta-lactams. Infect Dis Clin North Am. 1998; 11(4):875-87. DOI: 10.1016/s0891-5520(05)70395-0. View

16.

Yang Y, Bhachech N, Bradford P, Jett B, Sahm D, Bush K . Ceftazidime-resistant Klebsiella pneumoniae and Escherichia coli isolates producing TEM-10 and TEM-43 beta-lactamases from St. Louis, Missouri. Antimicrob Agents Chemother. 1998; 42(7):1671-6. PMC: 105664. DOI: 10.1128/AAC.42.7.1671. View

17.

Perilli M, Segatore B, de Massis M, Riccio M, Bianchi C, Zollo A . TEM-72, a new extended-spectrum beta-lactamase detected in Proteus mirabilis and Morganella morganii in Italy. Antimicrob Agents Chemother. 2000; 44(9):2537-9. PMC: 90100. DOI: 10.1128/AAC.44.9.2537-2539.2000. View