DNA Methylation Analysis by MethyLight Technology

Overview

Journal Methods

Specialty Biochemistry

Date 2002 Feb 16

PMID 11846615

Citations 53

Authors

B N Trinh

T I Long

P W Laird

Affiliations

Soon will be listed here.

Abstract

MethyLight is a sensitive, fluorescence-based real-time PCR technique that is capable of quantitating DNA methylation at a particular locus by using DNA oligonucleotides that anneal differentially to bisulfite-converted DNA according to the methylation status in the original genomic DNA. The use of three oligonucleotides (forward and reverse primers, and interpositioned probe) in MethyLight, any one or more of which can be used for methylation discrimination, allows for a high degree of specificity, sensitivity, and flexibility in methylation detection.

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