Amino Acid Sequence of an Immunoglobulin-like HLA Antigen Heavy Chain Domain

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1979 Nov 1

PMID 118453

Citations 6

Authors

L Tragardh

L Rask

K Wiman

J Fohlman

P A Peterson

Affiliations

Soon will be listed here.

Abstract

The classical human transplantation antigens, derived from the HLA-A, -B, and -C loci, are cell-surface-expressed glycoproteins. On the exterior of the cell the transplantation antigen heavy chain exposes two disulfide-containing domains and a glycosylated NH2-terminal extension. The disulfide-containing domain closest to the membrane has been isolated and its amino acid sequence has been determined. The HLA antigens used for the sequence analysis were derived from two and possibly three loci and comprised several allelic forms. The primary structure was remarkably invariant, and amino acid variations were observed only at three positions. Whether this suggests that the allelic variation of the HLA antigens is preferentially confined to other regions of the molecule or is a result of fortuitous selection of peptides remains to be established. The sequenced portion of the HLA antigen heavy chain is as homologous to beta 2-microglobulin and immunoglobulin light and heavy chains as are the latter to one another. This observation strengthens the notion that the transplantation antigens and the immunoglobulins are evolutionarily related.

Citing Articles

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Complete amino acid sequence of an HLA-DR antigen-like beta chain as predicted from the nucleotide sequence: similarities with immunoglobulins and HLA-A, -B, and -C antigens.

Larhammar D, Schenning L, Gustafsson K, Wiman K, Claesson L, Rask L Proc Natl Acad Sci U S A. 1982; 79(12):3687-91.

PMID: 6954511 PMC: 346491. DOI: 10.1073/pnas.79.12.3687.

Complete amino acid sequence of pooled papain-solubilized HLA-A, -B, and -C antigens: relatedness to immunoglobulins and internal homologies.

Tragardh L, Rask L, Wiman K, Fohlman J, Peterson P Proc Natl Acad Sci U S A. 1980; 77(2):1129-33.

PMID: 6928663 PMC: 348438. DOI: 10.1073/pnas.77.2.1129.

Two allelic forms of mouse beta 2-microglobulin.

Robinson P, Graf L, Sege K Proc Natl Acad Sci U S A. 1981; 78(2):1167-70.

PMID: 6165007 PMC: 319968. DOI: 10.1073/pnas.78.2.1167.

Allelic variation in HLA-B and HLA-C sequences and the evolution of the HLA-B alleles.

Pohla H, Kuon W, Tabaczewski P, Doerner C, Weiss E Immunogenetics. 1989; 29(5):297-307.

PMID: 2714852 DOI: 10.1007/BF00352839.

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