Urinary Levels of Monocyte Chemoattractant Protein-1 (MCP-1) and Interleukin-8 (IL-8), and Renal Injuries in Patients with Type 2 Diabetic Nephropathy

Overview

Journal J Clin Lab Anal

Publisher Wiley

Specialties Biomedical Engineering
Biotechnology
Pathology

Date 2002 Feb 9

PMID 11835523

Citations 48

Authors

Kyouichi Tashiro

Ichiro Koyanagi

Akemi Saitoh

Ayumi Shimizu

Toshihide Shike

Chizuru Ishiguro

Michiko Koizumi

Kazuhiko Funabiki

Satoshi Horikoshi

Isao Shirato

Yasuhiko Tomino

Affiliations

Soon will be listed here.

Abstract

We examined the correlation among the levels of urinary monocyte chemoattractant protein-1 (MCP-1) and interleukin-8 (IL-8), hyperglycemia, and renal injuries in patients with type 2 diabetic nephropathy. The levels of urinary MCP-1, IL-8, protein excretion, blood urea nitrogen (BUN), serum creatinine (s-Cr), glycohemoglobin A1c (HbA1c), and fasting plasma glucose (FPG) were measured in 24 patients with type 2 diabetic nephropathy and 14 healthy adults as controls. Diabetic nephropathy was classified into three stages: stage 1 = normoalbuminuric, stage 2 = microalbuminuric, and stage 3 = macroalbuminuric. All of the patients showed normal ranges in renal function tests. Levels of urinary MCP-1 in all patients with diabetic nephropathy were significantly higher than those in healthy adults (P < 0.05). The levels of urinary MCP-1 in patients with diabetic nephropathy increased gradually according to the clinical stage of this disease. In contrast, the levels of urinary IL-8 in patients with diabetic nephropathy increased in stages 2 and 3. There was a significant correlation between the levels of urinary IL-8 and those of HbA1c. High glucose may stimulate MCP-1 and/or IL-8 production and their excretion into the urine independently of the phases or pathological lesions of this disease. It appears that IL-8 increased in the early stage of diabetic nephropathy, and MCP-1 increased in the advanced stage of this disease. It was concluded that measurement of urinary MCP-1 and IL-8 may be useful for evaluating the degree of renal injuries in patients with type 2 diabetic nephropathy.

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References

Rovin B, Doe N, Tan L . Monocyte chemoattractant protein-1 levels in patients with glomerular disease. Am J Kidney Dis. 1996; 27(5):640-6. DOI: 10.1016/s0272-6386(96)90097-9. View

Wada T, Yokoyama H, Tomosugi N, Hisada Y, Ohta S, Naito T . Detection of urinary interleukin-8 in glomerular diseases. Kidney Int. 1994; 46(2):455-60. DOI: 10.1038/ki.1994.293. View

Saitoh A, Suzuki Y, Takeda M, Kubota K, Itoh K, Tomino Y . Urinary levels of monocyte chemoattractant protein (MCP)-1 and disease activity in patients with IgA nephropathy. J Clin Lab Anal. 1998; 12(1):1-5. PMC: 6808122. DOI: 10.1002/(sici)1098-2825(1998)12:1<1::aid-jcla1>3.0.co;2-f. View

Furuta T, Saito T, Ootaka T, Soma J, Obara K, Abe K . The role of macrophages in diabetic glomerulosclerosis. Am J Kidney Dis. 1993; 21(5):480-5. DOI: 10.1016/s0272-6386(12)80393-3. View

Brown Z, Strieter R, Chensue S, Ceska M, Lindley I, Neild G . Cytokine-activated human mesangial cells generate the neutrophil chemoattractant, interleukin 8. Kidney Int. 1991; 40(1):86-90. DOI: 10.1038/ki.1991.184. View

Young B, Johnson R, Alpers C, Eng E, Gordon K, Floege J . Cellular events in the evolution of experimental diabetic nephropathy. Kidney Int. 1995; 47(3):935-44. DOI: 10.1038/ki.1995.139. View

Yoshimura T, Matsushima K, Tanaka S, Robinson E, Appella E, Oppenheim J . Purification of a human monocyte-derived neutrophil chemotactic factor that has peptide sequence similarity to other host defense cytokines. Proc Natl Acad Sci U S A. 1987; 84(24):9233-7. PMC: 299727. DOI: 10.1073/pnas.84.24.9233. View

Gerritsma J, Hiemstra P, Gerritsen A, Prodjosudjadi W, Verweij C, van Es L . Regulation and production of IL-8 by human proximal tubular epithelial cells in vitro. Clin Exp Immunol. 1996; 103(2):289-94. PMC: 2200338. DOI: 10.1046/j.1365-2249.1996.d01-617.x. View

Stahl R, Thaiss F, Disser M, Helmchen U, Hora K, Schlondorff D . Increased expression of monocyte chemoattractant protein-1 in anti-thymocyte antibody-induced glomerulonephritis. Kidney Int. 1993; 44(5):1036-47. DOI: 10.1038/ki.1993.346. View

10.

Yokoyama H, Wada T, Furuichi K, Segawa C, Shimizu M, Kobayashi K . Urinary levels of chemokines (MCAF/MCP-1, IL-8) reflect distinct disease activities and phases of human IgA nephropathy. J Leukoc Biol. 1998; 63(4):493-9. DOI: 10.1002/jlb.63.4.493. View

11.

Bian Z, Elner S, Strieter R, Glass M, Lukacs N, Kunkel S . Glycated serum albumin induces chemokine gene expression in human retinal pigment epithelial cells. J Leukoc Biol. 1996; 60(3):405-14. DOI: 10.1002/jlb.60.3.405. View

12.

Wada T, Furuichi K, Sakai N, Iwata Y, Yoshimoto K, Shimizu M . Up-regulation of monocyte chemoattractant protein-1 in tubulointerstitial lesions of human diabetic nephropathy. Kidney Int. 2000; 58(4):1492-9. DOI: 10.1046/j.1523-1755.2000.00311.x. View

13.

Satriano J, Hora K, Shan Z, Stanley E, Mori T, Schlondorff D . Regulation of monocyte chemoattractant protein-1 and macrophage colony-stimulating factor-1 by IFN-gamma, tumor necrosis factor-alpha, IgG aggregates, and cAMP in mouse mesangial cells. J Immunol. 1993; 150(5):1971-8. View

14.

Oppenheim J, Zachariae C, Mukaida N, Matsushima K . Properties of the novel proinflammatory supergene "intercrine" cytokine family. Annu Rev Immunol. 1991; 9:617-48. DOI: 10.1146/annurev.iy.09.040191.003153. View

15.

Banba N, Nakamura T, Matsumura M, Kuroda H, Hattori Y, Kasai K . Possible relationship of monocyte chemoattractant protein-1 with diabetic nephropathy. Kidney Int. 2000; 58(2):684-90. DOI: 10.1046/j.1523-1755.2000.00214.x. View

16.

Sekizuka K, Tomino Y, Sei C, Kurusu A, Tashiro K, Yamaguchi Y . Detection of serum IL-6 in patients with diabetic nephropathy. Nephron. 1994; 68(2):284-5. DOI: 10.1159/000188281. View

17.

Wada T, Yokoyama H, Su S, Mukaida N, Iwano M, Dohi K . Monitoring urinary levels of monocyte chemotactic and activating factor reflects disease activity of lupus nephritis. Kidney Int. 1996; 49(3):761-7. DOI: 10.1038/ki.1996.105. View

18.

Baggiolini M, DEWALD B, Moser B . Interleukin-8 and related chemotactic cytokines--CXC and CC chemokines. Adv Immunol. 1994; 55:97-179. View

19.

Wada T, Furuichi K, Shimizu M, Sakai N, Takeda S, Takasawa K . MIP-1alpha and MCP-1 contribute to crescents and interstitial lesions in human crescentic glomerulonephritis. Kidney Int. 1999; 56(3):995-1003. DOI: 10.1046/j.1523-1755.1999.00646.x. View

20.

Ihm C, Park J, Hong S, Lee T, Cho B, Kim M . A high glucose concentration stimulates the expression of monocyte chemotactic peptide 1 in human mesangial cells. Nephron. 1998; 79(1):33-7. DOI: 10.1159/000044988. View