Neutralizing and Complement-fixing Monoclonal Antibodies As an Aid to the Diagnosis of Equine Herpesvirus-1 Infection

Overview

Journal Vet Res Commun

Publisher Springer

Specialty Veterinary Medicine

Date 2002 Jan 5

PMID 11767013

Citations 1

Authors

B K Singh

M P Yadav

S C Tewari

Affiliations

Soon will be listed here.

Abstract

One complement-fixing (C-MAb) and three complement-dependent neutralizing monoclonal antibodies (N-MAbs) were raised against Hisar-90-7 equine herpesvirus-1 (EHV-1) strain. The target antigen of the C-MAb (2A5) and two of the N-MAbs (1H6, 9C4) was identified as a 140 kDa polypeptide in Western blotting. The target antigen of N-MAb (9C6) could not be identified. Purified polypeptides of five EHV-1 strains isolated from different regions and at different times gave intense bands at 140 kDa when reacted with N-MAb (1H6) in Western blots. In sandwich ELISA, all four MAbs captured the viral antigen from clinical materials, giving a reliable and rapid diagnosis of EHV-1 infection in equines.

Citing Articles

Development of a neutralizing monoclonal antibody-based blocking ELISA for detection of equine herpesvirus 1 antibodies.

Singh B, Ahuja S, Gulati B Vet Res Commun. 2004; 28(5):437-46.

PMID: 15379438 DOI: 10.1023/b:verc.0000034996.18533.90.

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