Utilization of Ribonucleic Acid and Deoxyoligomer Primers for Polyadenylic Acid Synthesis by Adenosine Triphosphate: Polynucleotidylexotransferase from Maize

Overview

Journal J Biol Chem

Specialty Biochemistry

Date 1975 May 25

PMID 1168636

Citations 8

Authors

R J Mans

N J Huff

Affiliations

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Abstract

The ATP:polynucleotidylexotransferase isolated and purified from maize seedlings catalyzes the synthesis of polyadenylic acid by the sequential addition of 80 to 200 AMP moieties from ATP to the 3'-hydroxyl terminus of either ribo- or deoxyoligomers. Copurification of the RNA and DNA-primed activities, identical metal cofactor and reaction requirements for either primer and identical heat inactivation curves with either primer strongly suggest that both primers are utilized by the same enzyme.

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