Eukaryotic Elongation Factor 2 Can Bind to the Synthetic Oligoribonucleotide That Mimics Sarcin/ricin Domain of Rat 28S Ribosomal RNA

Overview

Journal Mol Cell Biochem

Publisher Springer

Specialty Biochemistry

Date 2001 Oct 30

PMID 11681712

Citations 1

Authors

S Tang

W J He

H Xu

W Y Liu

K C Ruan

Affiliations

Soon will be listed here.

Abstract

Eukaryotic elongation factor 2 (eEF2) catalyzes the translocation of peptidyl-tRNA from the A site to P site by binding to the ribosome. In this work, the complex formation of rat liver eEF2 with a synthetic oligoribonucleotide (SRD RNA) that mimics sarcin/ricin domain of rat 28S ribosomal RNA is invested in vitro. Purified eEF2 can specifically bind SRD RNA to form a stable complex. tRNA competes with SRD RNA in binding to eEF2 in a less extent. Pretreatment of eEF2 with GDP or ADP-ribosylation of eEF2 by diphtheria toxin can obviously reduce the ability of eEF2 to form the complex with the synthetic oligoribonucleotide. These results indicate that eEF2 is likely to bind directly to the sarcin/ricin domain of 28S ribosomal RNA in the process of protein synthesis.

Citing Articles

Modes of action of ADP-ribosylated elongation factor 2 in inhibiting the polypeptide elongation cycle: a modeling study.

Chen K, Xie H, Cai Y PLoS One. 2013; 8(7):e66446.

PMID: 23861744 PMC: 3704607. DOI: 10.1371/journal.pone.0066446.

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