K+-conducting Ion Channel of the Chloroplast Inner Envelope: Functional Reconstitution into Liposomes

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1993 Jun 1

PMID 11607404

Citations 9

Authors

X Wang

G A Berkowitz

J S Peters

Affiliations

Soon will be listed here.

Abstract

Potassium flux between the chloroplast stroma and cytoplasm is known to be indirectly linked to H+ countertransport and, hence, stromal pH and photosynthetic capacity. The specific molecular mechanism that facilitates K+ flux across the chloroplast envelope is not known and has been a source of controversy for well over a decade. The objective of this study was to elucidate the nature of this envelope protein. To this end, solubilized protein in detergent extracts of purified chloroplast inner envelope vesicles was reconstituted into artificial liposomes, and cation fluxes into these proteoliposomes were measured. Results of inhibitor studies and counterflux experiments indicated that a K+-conducting ion channel was solubilized and functionally reconstituted into the proteoliposomes. This transport protein may be a nonspecific monovalent cation channel. This report represents a direct demonstration of ion channel activity associated with the limiting (inner) membrane of the chloroplast envelope.

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