Lipid Modification of the Cu,Zn Superoxide Dismutase from Mycobacterium Tuberculosis

Overview

Journal Biochem J

Specialty Biochemistry

Date 2001 Sep 21

PMID 11563965

Citations 17

Authors

M DOrazio

S Folcarelli

F Mariani

V Colizzi

G Rotilio

A Battistoni

Affiliations

Soon will be listed here.

Abstract

The leader sequence of Mycobacterium tuberculosis Cu,Zn superoxide dismutase (Cu,ZnSOD) contains a prokaryotic membrane lipoprotein attachment site. In the present study, we have found that the protein, which exhibits detectable SOD activity, is lipid-modified and associated with the bacterial membrane when expressed either in M. tuberculosis or in Escherichia coli. These results provide the first demonstration of lipid modification of a Cu,ZnSOD. An analysis of the sodC genes present in available databases indicates that the same signal for lipid modification is also present in the sodC gene products from other mycobacteria and Gram-positive bacteria and, uniquely, in two distinct sodC gene products from the Gram-negative bacterium Salmonella typhimurium. Evidence is also provided for an up-regulation of M. tuberculosis sodC in response to phagocytosis by human macrophages, suggesting that Cu,ZnSOD is involved in the mechanisms that facilitate mycobacterial intracellular growth.

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