Low Sensitivity of Polymerase Chain Reaction for Diagnosis of Tuberculous Meningitis in Southeastern Brazil

Overview

Journal Rev Soc Bras Med Trop

Specialty Tropical Medicine

Date 2001 Sep 20

PMID 11562736

Citations 8

Authors

V M Brienze

Tonon AP

F J Pereira

E Liso

W A Tognola

M A Dos Santos

M U Ferreira

Affiliations

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Abstract

Two polymerase chain reaction (PCR) protocols showed low sensitivity (36% and 53% for TB AMPLICOR and MPB64 nested PCR, respectively), when compared with classic microbiological methods (73% and 54% for Ziehl-Neelsen staining and culture, respectively), in the diagnosis of tuberculous meningitis in 91 patients in southeastern Brazil. Only three PCR-positive, microbiologically negative patients were found. Analysis of sequential cerebrospinal fluid samples by nested PCR detected Mycobacterium tuberculosis DNA up to 29 days after the introduction of antituberculosis chemotherapy.

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