Clinical Value of ELISA-MPT64 for the Diagnosis of Tuberculous Pleurisy

Overview

Journal Curr Microbiol

Specialty Microbiology

Date 2012 Jun 8

PMID 22674175

Citations 5

Authors

Zhonghua Liu

Changtai Zhu

Hua Yang

Haili Hu

Yonghong Feng

Lianhua Qin

Zhenling Cui

Aixiao Bi

Ruijuan Zheng

Ruiliang Jin

Lin Fan

Zhongyi Hu

Affiliations

Soon will be listed here.

Abstract

Tuberculous pleurisy is one of the common extrapulmonary tuberculosis diseases. However, the diagnosis of tuberculous pleurisy still lacks a useful and effective tool, mainly due to paucity of Mycobacterium tuberculosis organisms in pleural effusion. Previous studies have confirmed that the MPT64 protein is highly specific and is secreted only by M. tuberculosis (MTB) complex. Therefore, in this study, we developed ELISA based on recombinantly expressed MPT64 in combination with rabbit polyclonal antibodies. The ELISA-MPT64 method was validated using MTB strains and tested against clinical samples. Nested PCR, Löwenstein-Jensen (L-J) culture and smear microscopy were employed as the comparative tools for assessing the performance of the assay. Our results demonstrate that the newly established ELISA-MPT64 technique is a rapid and useful tool for the diagnosis of tuberculous pleurisy.

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