» Articles » PMID: 1150665

The Primary Structure of Actin from Rabbit Skeletal Muscle. Completion and Analysis of the Amino Acid Sequence

Overview
Journal J Biol Chem
Specialty Biochemistry
Date 1975 Aug 10
PMID 1150665
Citations 58
Authors
Affiliations
Soon will be listed here.
Abstract

Actin is the principal constituent of the thin filaments of muscle, and in order to provide information basic to understanding the molecular basis of actin function we have studied its amino acid sequence. The isolation, compositions, and sequences of cyanogen bromide peptides, ranging in size from 3 to 44 residues, have previously been reported (ELZINGA, M. (1971) Biochemistry 10, 224-229, and other papers in the present series). The peptides have been aligned by isolation and characterization of tryptic peptides that contain methionine. The isolation of one of the CNBr peptides (CB-14) was complicated by the presence of a Met-Thr bond that was only partially split under standard conditions for cyanogen bromide cleavage in formic acid. In this paper conditions are described for increasing the cleavage at this bond. CB-14 is a tetrapeptide, Thr-Gln-Ile-Hse, and this sequence completes the characterization of the actin cyanogen bromide peptides. Finally, the position of CB-14 in the actin sequence as residues 120 to 123 was established by isolation of a chymotryptic overlap peptide. The complete sequence of the 374 residues of actin is presented.

Citing Articles

Purification of modified mammalian actin isoforms for in vitro reconstitution assays.

Kast D, Jansen S Eur J Cell Biol. 2023; 102(4):151363.

PMID: 37778219 PMC: 10872616. DOI: 10.1016/j.ejcb.2023.151363.


Algal Toxin Goniodomin A Binds Potassium Ion Selectively to Yield a Conformationally Altered Complex with Potential Biological Consequences.

Tainter C, Schley N, Harris C, Stec D, Song A, Balinski A J Nat Prod. 2020; 83(4):1069-1081.

PMID: 32083860 PMC: 9290314. DOI: 10.1021/acs.jnatprod.9b01094.


Actin filaments-A target for redox regulation.

Wilson C, Terman J, Gonzalez-Billault C, Ahmed G Cytoskeleton (Hoboken). 2016; 73(10):577-595.

PMID: 27309342 PMC: 5069131. DOI: 10.1002/cm.21315.


BD SIMULATIONS OF THE IONIC STRENGTH DEPENDENCE OF THE INTERACTIONS BETWEEN TRIOSE PHOSPHATE ISOMERASE AND F-ACTIN.

Schmidt E, Forlemu N, Njabon E, Thomasson K J Undergrad Chem Res. 2014; 9(4):87-96.

PMID: 24639622 PMC: 3955172.


Analysis of Flavonoids from Eugenia uniflora Leaves and Its Protective Effect against Murine Sepsis.

Rattmann Y, de Souza L, Malquevicz-Paiva S, Dartora N, Sassaki G, Gorin P Evid Based Complement Alternat Med. 2013; 2012:623940.

PMID: 23320032 PMC: 3539388. DOI: 10.1155/2012/623940.