Fluorometrical Detection of Thymine Base Differences in Complementary Strands of Satellite DNA in Human Metaphase Chromosomes

Overview

Journal Humangenetik

Specialty Genetics

Date 1975 May 26

PMID 1150260

Citations 7

Authors

M A Kim

Affiliations

Soon will be listed here.

Abstract

Using the fluorochrome "Hoechst 33258", intensity of fluorescence was found to differ distinctly between the sister chromatids in the paracentric regions of chromosomes 1, 16, and 19, after one round of replication in medium containing BUdR. Thus the effect of fluorescence asymmetry is not limited to the part of the Y chromosomes that fluoresces intensely with quinacrine; it can also be determined in the weakly Q-fluorescent pericentric regions of chromosomes, which are known to be the sites where highly reiterated sequences of satellite DNA are located. However, an exception is the paracentric region of chromosome 9 which does not show the effect of lateral asymmetry. The difference of fluorescence intensity in the heterochromatic regions of the sister chromatids of human chromosome 1 is measured by densitometric tracement along the long axes of chromosomes; this is obtained from two individuals with an "uncoiler" heterchomatic block (type III) having a relative intensity of 1:1.93 in an average of the total measured blocks. This corresponds to the uneven distribution of thymine base of 22.8 and 43.2 in the two strands of the DNA double hexlix. A chromatid exchange rate of 9 in 100 metaphases per cell cycle was found within the uncoiler region of chromosome 1.

Citing Articles

Compound lateral asymmetry in human chromosome 6:BrdU-dye studies of 6q12-->6q14.

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On the origin of lateral asymmetry.

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Aspects of evaluation, significance, and evolution of human C-band heteromorphism.

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Lateral asymmetry in human constitutive heterochromatin: frequency and inheritance.

Angell R, Jacobs P Am J Hum Genet. 1978; 30(2):144-52.

PMID: 566032 PMC: 1685568.

Lateral asymmetry of constitutive heterochromatin in human chromosomes.

Ghosh P, Rani R, Nand R Hum Genet. 1979; 52(1):79-84.

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