Mutant of the Yeast Saccharomycopsis Lipolytica That Accumulates and Excretes Protorphyrin IX

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1975 Jul 1

PMID 1141193

Citations 5

Authors

J Bassel

P Hambright

R Mortimer

A J Bearden

Affiliations

Soon will be listed here.

Abstract

The red, water-insoluble pigment excreted by a mutant strain of the yeast Saccharomycopsis lipolytica is show to be protoporphyrin IX. In genetic crosses the red phenotype has the properties characteristic of a defect in a single, recessive nuclear gene. The yield and ease of harvest of protoporphyrin IX from the yeast mutant indicate that this strain or its derivatives may be a valuable source of this substance.

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References

BASES R, BRODIE S, RUBENFELD S . Attempts at tumor localization using Cu 64-labeled copper porphyrins. Cancer. 1958; 11(2):259-63. DOI: 10.1002/1097-0142(195803/04)11:2<259::aid-cncr2820110206>3.0.co;2-2. View

Yarrow D . Four new combinations in yeasts. Antonie Van Leeuwenhoek. 1972; 38(3):357-60. DOI: 10.1007/BF02328105. View

Bassel J, Mortimer R . Genetic analysis of mating type and alkane utilization in Saccharomycopsis lipolytica. J Bacteriol. 1973; 114(2):894-6. PMC: 251857. DOI: 10.1128/jb.114.2.894-896.1973. View

Fawwaz R, Frye F, LOUGHMAN W, HEMPHILL W . Survival of skin homografts in dogs injected with 109Pd-protoporphyrin. J Nucl Med. 1974; 15(11):997-1002. View

Cox R, CHARLES H . Porphyrin-accumulating mutants of Escherichia coli. J Bacteriol. 1973; 113(1):122-32. PMC: 251610. DOI: 10.1128/jb.113.1.122-132.1973. View

Bassel J, Warfel J, Mortimer R . Complementation and genetic recombination in Candida lipolytica. J Bacteriol. 1971; 108(1):609-11. PMC: 247111. DOI: 10.1128/jb.108.1.609-611.1971. View

Fawwaz R, HEMPHILL W, WINCHELL H . Potential use of 109Pd-porphyrin complexes for selective lymphatic ablation. J Nucl Med. 1971; 12(5):231-6. View

Wickerham L, Kurtzman C, Herman A . Sexual reproduction in Candida lipolytica. Science. 1970; 167(3921):1141. DOI: 10.1126/science.167.3921.1141. View

LASCELLES J, Hatch T . Bacteriochlorophyll and heme synthesis in Rhodopseudomonas spheroides: possible role of heme in regulation of the branched biosynthetic pathway. J Bacteriol. 1969; 98(2):712-20. PMC: 284876. DOI: 10.1128/jb.98.2.712-720.1969. View

10.

Miyake S, Sugimura T . Coproporphyrinogenase in a respiration-deficient mutant of yeast lacking all cytochromes and accumulating coproporphyrin. J Bacteriol. 1968; 96(6):1997-2003. PMC: 252550. DOI: 10.1128/jb.96.6.1997-2003.1968. View

11.

Winkelman J, Slater G, Grossman J . The concentration in tumor and other tissues of parenterally administered tritium- and 14-C-labeled tetraphenylporphinesulfonate. Cancer Res. 1967; 27(11):2060-4. View