Identification of a 170-kDa Protein Over-expressed in Lung Cancers

Overview

Journal Br J Cancer

Specialty Oncology

Date 2001 Jun 1

PMID 11384103

Citations 44

Authors

R Pincheira

Q Chen

J T Zhang

Affiliations

Soon will be listed here.

Abstract

Lung cancer is the leading cause for cancer death in both male and female populations. Although many molecular markers for lung cancer have been developed and useful for early detection of lung cancer, their function remains unknown. In this paper, we report our findings that a 170-kDa protein (p170) is over-expressed in all types of human lung cancers compared with normal tissues and it is identified as a subunit of translation initiation factor eIF3 by cDNA cloning. Translation initiation factors are a family of proteins that promote the initiation step of protein synthesis and are regulators of cell growth at the translational level. Further studies showed that p170 mRNA is ubiquitously expressed with higher levels in adult proliferating tissues (e.g. bone marrow) and tissues during development (e.g. fetal tissues). This study suggests that p170 and eIF3 may be important factors for cell growth, development, and tumorigenesis.

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References

Trachsel H, Erni B, SCHREIER M, Staehelin T . Initiation of mammalian protein synthesis. II. The assembly of the initiation complex with purified initiation factors. J Mol Biol. 1977; 116(4):755-67. DOI: 10.1016/0022-2836(77)90269-8. View

Zhou J, Mulshine J, Unsworth E, Scott F, Avis I, Vos M . Purification and characterization of a protein that permits early detection of lung cancer. Identification of heterogeneous nuclear ribonucleoprotein-A2/B1 as the antigen for monoclonal antibody 703D4. J Biol Chem. 1996; 271(18):10760-6. DOI: 10.1074/jbc.271.18.10760. View

Riordan J, Ling V . Purification of P-glycoprotein from plasma membrane vesicles of Chinese hamster ovary cell mutants with reduced colchicine permeability. J Biol Chem. 1979; 254(24):12701-5. View

Goumans H, Thomas A, Verhoeven A, VOORMA H, Benne R . The role of eIF-4C in protein synthesis initiation complex formation. Biochim Biophys Acta. 1980; 608(1):39-46. DOI: 10.1016/0005-2787(80)90131-8. View

Meyer L, Corbett S, Tolan D, Hershey J . The purification and characterization of multiple forms of protein synthesis eukaryotic initiation factors 2, 3, and 5 from rabbit reticulocytes. J Biol Chem. 1981; 256(1):351-6. View

Harbeck R, Hoffman A, Redecker S, Biundo T, Kurnick J . The isolation and functional activity of polymorphonuclear leukocytes and lymphocytes separated from whole blood on a single percoll density gradient. Clin Immunol Immunopathol. 1982; 23(3):682-90. DOI: 10.1016/0090-1229(82)90331-2. View

Meyer L, Milburn S, Hershey J . Immunochemical characterization of mammalian protein synthesis initiation factors. Biochemistry. 1982; 21(18):4206-12. DOI: 10.1021/bi00261a003. View

Georges E, Bradley G, Gariepy J, Ling V . Detection of P-glycoprotein isoforms by gene-specific monoclonal antibodies. Proc Natl Acad Sci U S A. 1990; 87(1):152-6. PMC: 53218. DOI: 10.1073/pnas.87.1.152. View

Montine K, Sonenberg N . Malignant transformation by a eukaryotic initiation factor subunit that binds to mRNA 5' cap. Nature. 1990; 345(6275):544-7. DOI: 10.1038/345544a0. View

10.

Geiser J, Shu M, Miller C, Wool I, Meisler A, Pipas J . Ribosomal protein genes are overexpressed in colorectal cancer: isolation of a cDNA clone encoding the human S3 ribosomal protein. Mol Cell Biol. 1991; 11(8):3842-9. PMC: 361167. DOI: 10.1128/mcb.11.8.3842-3849.1991. View

11.

Zhang M, Wang G, Shapiro A, Zhang J . Topological folding and proteolysis profile of P-glycoprotein in membranes of multidrug-resistant cells: implications for the drug-transport mechanism. Biochemistry. 1996; 35(30):9728-36. DOI: 10.1021/bi960400s. View

12.

Methot N, Song M, Sonenberg N . A region rich in aspartic acid, arginine, tyrosine, and glycine (DRYG) mediates eukaryotic initiation factor 4B (eIF4B) self-association and interaction with eIF3. Mol Cell Biol. 1996; 16(10):5328-34. PMC: 231531. DOI: 10.1128/MCB.16.10.5328. View

13.

Kho C, Wang Y, Zarbl H . Effect of decreased fte-1 gene expression on protein synthesis, cell growth, and transformation. Cell Growth Differ. 1996; 7(9):1157-66. View

14.

Asano K, Kinzy T, Merrick W, Hershey J . Conservation and diversity of eukaryotic translation initiation factor eIF3. J Biol Chem. 1997; 272(2):1101-9. DOI: 10.1074/jbc.272.2.1101. View

15.

Methot N, Rom E, Olsen H, Sonenberg N . The human homologue of the yeast Prt1 protein is an integral part of the eukaryotic initiation factor 3 complex and interacts with p170. J Biol Chem. 1997; 272(2):1110-6. DOI: 10.1074/jbc.272.2.1110. View

16.

Bachmann F, Banziger R, Burger M . Cloning of a novel protein overexpressed in human mammary carcinoma. Cancer Res. 1997; 57(5):988-94. View

17.

Johnson K, Merrick W, Zoll W, Zhu Y . Identification of cDNA clones for the large subunit of eukaryotic translation initiation factor 3. Comparison of homologues from human, Nicotiana tabacum, Caenorhabditis elegans, and Saccharomyces cerevisiae. J Biol Chem. 1997; 272(11):7106-13. DOI: 10.1074/jbc.272.11.7106. View

18.

Scholler J, Kanner S . The human p167 gene encodes a unique structural protein that contains centrosomin A homology and associates with a multicomponent complex. DNA Cell Biol. 1997; 16(4):515-31. DOI: 10.1089/dna.1997.16.515. View

19.

Carbone D . The biology of lung cancer. Semin Oncol. 1997; 24(4):388-401. View

20.

Asano K, Merrick W, Hershey J . The translation initiation factor eIF3-p48 subunit is encoded by int-6, a site of frequent integration by the mouse mammary tumor virus genome. J Biol Chem. 1997; 272(38):23477-80. DOI: 10.1074/jbc.272.38.23477. View