Inhibition of L-deleted Foot-and-mouth Disease Virus Replication by Alpha/beta Interferon Involves Double-stranded RNA-dependent Protein Kinase

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 2001 May 18

PMID 11356957

Citations 57

Authors

J Chinsangaram

M Koster

M J Grubman

Affiliations

Soon will be listed here.

Abstract

We previously demonstrated that the ability of foot-and-mouth disease virus (FMDV) to form plaques in cell culture is associated with the suppression of alpha/beta interferon (IFN-alpha/beta). In the present study, we used Escherichia coli-expressed porcine and bovine IFN-alpha or -beta individually to demonstrate that each was equally effective in inhibiting FMDV replication. The block in FMDV replication appeared to be at the level of protein translation, suggesting a role for double-stranded RNA-dependent protein kinase (PKR). In support of these findings, treatment of porcine and bovine cells with 2-aminopurine, an inhibitor of PKR, increased the yield of virus 8.8- and 11.2-fold, respectively, compared to that in untreated infected cells. In addition, results of FMDV infection in mouse embryonic fibroblast cells derived from gene knockout mice lacking the gene for RNase L(-/-) or PKR(-/-) or both indicated an important role for PKR in the inhibition of FMDV replication.

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