Homologous Recombination in Planta is Stimulated in the Absence of Rad50

Overview

Journal EMBO Rep

Specialty Molecular Biology

Date 2001 Apr 18

PMID 11306548

Citations 44

Authors

H Gherbi

M E Gallego

N Jalut

J M Lucht

B Hohn

C I White

Affiliations

Soon will be listed here.

Abstract

Chromosomal double-strand DNA breaks must be repaired; in the absence of repair the resulting acentromeric (and telomereless) fragments may be lost and/or the broken DNA ends may recombine causing general chromosomal instability. The Rad50/Mre11/Xrs2 protein complex acts at DNA ends and is implicated in both homologous and non-homologous recombination. We have isolated a rad50 mutant of the plant Arabidopsis thaliana and show here that it has a somatic hyper-recombination phenotype in planta. This finding supports the hypothesis of a competition between homologous and illegitimate recombination in higher eukaryotes. To our knowledge, this is the first direct in vivo support for the role of this complex in chromosomal recombination in a multicellular organism and the first description of a mutation of a known gene leading to hyper-recombination in plants.

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References

Paques F, Haber J . Multiple pathways of recombination induced by double-strand breaks in Saccharomyces cerevisiae. Microbiol Mol Biol Rev. 1999; 63(2):349-404. PMC: 98970. DOI: 10.1128/MMBR.63.2.349-404.1999. View

Schiestl R, Zhu J, Petes T . Effect of mutations in genes affecting homologous recombination on restriction enzyme-mediated and illegitimate recombination in Saccharomyces cerevisiae. Mol Cell Biol. 1994; 14(7):4493-500. PMC: 358821. DOI: 10.1128/mcb.14.7.4493-4500.1994. View

Critchlow S, Jackson S . DNA end-joining: from yeast to man. Trends Biochem Sci. 1998; 23(10):394-8. DOI: 10.1016/s0968-0004(98)01284-5. View

Tsukamoto Y, Kato J, Ikeda H . Effects of mutations of RAD50, RAD51, RAD52, and related genes on illegitimate recombination in Saccharomyces cerevisiae. Genetics. 1996; 142(2):383-91. PMC: 1206973. DOI: 10.1093/genetics/142.2.383. View

Clough S, Bent A . Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J. 1999; 16(6):735-43. DOI: 10.1046/j.1365-313x.1998.00343.x. View

Britt . Molecular genetics of DNA repair in higher plants. Trends Plant Sci. 1999; 4(1):20-25. DOI: 10.1016/s1360-1385(98)01355-7. View

Xiao Y, Weaver D . Conditional gene targeted deletion by Cre recombinase demonstrates the requirement for the double-strand break repair Mre11 protein in murine embryonic stem cells. Nucleic Acids Res. 1997; 25(15):2985-91. PMC: 146850. DOI: 10.1093/nar/25.15.2985. View

Tsukamoto Y, Ikeda H . Double-strand break repair mediated by DNA end-joining. Genes Cells. 1998; 3(3):135-44. DOI: 10.1046/j.1365-2443.1998.00180.x. View

Mengiste T, Paszkowski J . Prospects for the precise engineering of plant genomes by homologous recombination. Biol Chem. 1999; 380(7-8):749-58. DOI: 10.1515/BC.1999.095. View

10.

Lewis L, Westmoreland J, Resnick M . Repair of endonuclease-induced double-strand breaks in Saccharomyces cerevisiae: essential role for genes associated with nonhomologous end-joining. Genetics. 1999; 152(4):1513-29. PMC: 1460701. DOI: 10.1093/genetics/152.4.1513. View

11.

Luo G, Yao M, Bender C, Mills M, Bladl A, Bradley A . Disruption of mRad50 causes embryonic stem cell lethality, abnormal embryonic development, and sensitivity to ionizing radiation. Proc Natl Acad Sci U S A. 1999; 96(13):7376-81. PMC: 22093. DOI: 10.1073/pnas.96.13.7376. View

12.

Malone R, Esposito R . Recombinationless meiosis in Saccharomyces cerevisiae. Mol Cell Biol. 1981; 1(10):891-901. PMC: 369377. DOI: 10.1128/mcb.1.10.891-901.1981. View

13.

Tinland B, Hohn B, Puchta H . Agrobacterium tumefaciens transfers single-stranded transferred DNA (T-DNA) into the plant cell nucleus. Proc Natl Acad Sci U S A. 1994; 91(17):8000-4. PMC: 44532. DOI: 10.1073/pnas.91.17.8000. View

14.

Gallego M, Jeanneau M, Granier F, Bouchez D, Bechtold N, White C . Disruption of the Arabidopsis RAD50 gene leads to plant sterility and MMS sensitivity. Plant J. 2001; 25(1):31-41. DOI: 10.1046/j.1365-313x.2001.00928.x. View

15.

Malone R, Ward T, Lin S, Waring J . The RAD50 gene, a member of the double strand break repair epistasis group, is not required for spontaneous mitotic recombination in yeast. Curr Genet. 1990; 18(2):111-6. DOI: 10.1007/BF00312598. View

16.

Milne G, Jin S, Shannon K, Weaver D . Mutations in two Ku homologs define a DNA end-joining repair pathway in Saccharomyces cerevisiae. Mol Cell Biol. 1996; 16(8):4189-98. PMC: 231416. DOI: 10.1128/MCB.16.8.4189. View

17.

Yamaguchi-Iwai Y, Sonoda E, Sasaki M, Morrison C, Haraguchi T, Hiraoka Y . Mre11 is essential for the maintenance of chromosomal DNA in vertebrate cells. EMBO J. 1999; 18(23):6619-29. PMC: 1171725. DOI: 10.1093/emboj/18.23.6619. View

18.

Gottlieb S, Wagstaff J, Esposito R . Evidence for two pathways of meiotic intrachromosomal recombination in yeast. Proc Natl Acad Sci U S A. 1989; 86(18):7072-6. PMC: 297996. DOI: 10.1073/pnas.86.18.7072. View

19.

Swoboda P, Gal S, Hohn B, Puchta H . Intrachromosomal homologous recombination in whole plants. EMBO J. 1994; 13(2):484-9. PMC: 394832. DOI: 10.1002/j.1460-2075.1994.tb06283.x. View

20.

Jeggo P . DNA breakage and repair. Adv Genet. 1998; 38:185-218. DOI: 10.1016/s0065-2660(08)60144-3. View